Process of synthesizing 6-methylpurine-2'-deoxyncleoside with gene engineering bacterium

A technology of genetically engineered bacteria and methyl purine, applied in genetic engineering, plant genetic improvement, botanical equipment and methods, etc., can solve problems such as difficult separation, a large number of isomers, chemical catalyst personnel and environmental poisoning, and achieve economical The effect of cost, short cycle and low cost
CN101067145AInactive Publication Date: 2007-11-07FUDAN UNIV
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FUDAN UNIV
Publication Date
2007-11-07
Estimated Expiration
Not applicable · inactive patent

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Abstract

The present invention belongs to the field of biomedicine preparing technology, and is especially the process of constructing gene engineering bacterium by means of DNA recombining technology and synthesizing 6-methylpurine-2'-deoxyriboside (MePdR) with the gene engineering bacterium. The process includes the following steps: 1. constructing purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase expressing vector, and transforming to colibacillus to obtain gene engineering bacterium with high efficiency expression of purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase; 2. catalyzing the reaction between pyrimidine deoxyriboside and 6-methylpurine with the purine nucleoside phosphorylase and pyrimidine nucleoside phosphorylase to synthesize MePdR; and 3. separating and purifying MePdR. The catalytic synthesis process of MePdR has the features of simplicity, high efficiency, ets, and is suitable for industrial production.
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Description

technical field

[0001] The invention belongs to the technical field of biopharmaceuticals, and specifically relates to a genetically engineered bacterium constructed by DNA recombination technology, and the genetically engineered bacterium is used to synthesize 6-methylpurine-2'-deoxyriboside (6-Methylpurine-2'-Deoxyriboside, MePdR) method. Background technique

[0002] MePdR is the prodrug of Escherichia coli PNP / 6-methylpurine-2′-deoxynucleoside (ePNP / MePdR) anti-tumor therapy system. 2.4.2.1, purinenucleoside phosphorylase, PNP) decomposes into 6-methylpurine (6-Methylpurine, MeP), MeP can inhibit RNA and protein synthesis, leading to cell death; mammalian PNPase cannot decompose MePdR. E.coli PNP gene is transferred to tumor cells to express E.coli PNP. When the drug precursor MePdR exists, E.coli PNP will decompose it into toxic MeP to kill tumor cells efficiently.

[0003] Compared with other suicide gene / prodrug anti-tumor therapeutic systems, the ePNP / MePdR anti-tu...

Claims

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