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Colloidal gold chromatographic band for detecting IgM, IgG antibody by double-capture method and producing method thereof

A technology of colloidal gold and capture method, applied in measuring devices, analytical materials, instruments, etc., can solve the problems of cumbersome operation process, short antibody half-life, inconvenient and fast detection, etc., and achieve the effect of high overall coincidence rate and simplified operation process

Active Publication Date: 2012-04-04
北京英诺特生物技术股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

If a specific IgM antibody is detected in the serum, it indicates that a recent infection has occurred, but the half-life of the IgM antibody is short, and a negative IgM test does not prove that the body is not infected. Sometimes it is necessary to detect the IgG antibody with a longer half-life and a higher content , to confirm the diagnosis
[0003] At present, the detection of specific IgM and IgG antibodies mainly adopts the method of separate detection and comprehensive analysis. First, conventional ELISA method, double antigen sandwich method or colloidal gold immunochromatography are used to detect IgM and IgG antibodies respectively, and then comprehensive analysis of the two The whole operation process is cumbersome, and the detection is not convenient and fast enough.

Method used

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  • Colloidal gold chromatographic band for detecting IgM, IgG antibody by double-capture method and producing method thereof
  • Colloidal gold chromatographic band for detecting IgM, IgG antibody by double-capture method and producing method thereof
  • Colloidal gold chromatographic band for detecting IgM, IgG antibody by double-capture method and producing method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: detect the IgM, IgG antibody of anti-human cytomegalovirus (HCMV)

[0020] 1, the preparation method of the colloidal gold chromatography strip that detects human cytomegalovirus (HCMV) IgM, IgG antibody

[0021] (1) The rabbit anti-HCMV antibody with a concentration of 5 mg / ml was prepared by animal immunization method, and the mouse anti-human IgM μ chain monoclonal antibody with a concentration of 2 mg / ml and the mouse anti-HCMV with a concentration of 4 mg / ml were respectively prepared by a monoclonal antibody preparation method. Human IgGγ chain monoclonal antibody, spare.

[0022] (2) Preparation of antigen-colloidal gold complex: Colloidal gold was prepared by trisodium citrate reduction method, and purified HCMV antigen was added to the gold sol at a ratio of 60 μg / ml. After sealing and centrifugation, the precipitate was diluted to A 530 Value 2.5, spare.

[0023] (3) Coating with mouse anti-human IgM μ chain monoclonal antibody, mouse anti-huma...

Embodiment 2

[0042] Embodiment 2: detect the IgM, IgG antibody of anti-rubella virus (RV)

[0043] A colloidal gold chromatography strip for detecting anti-rubella virus (RV) IgM and IgG antibodies was prepared by a method similar to that of Example 1. The prepared colloidal gold chromatography strip was used to detect 568 anti-RV IgG positive sera and 1563 negative sera with clear clinical diagnosis, and compared with the results of anti-RV IgG detected by indirect ELISA reagent alone. The results are shown in Table 5 and Table 6.

[0044] The detection result of table 5 colloidal gold chromatography strip anti-RV IgG clinical serum

[0045]

[0046] Table 6 colloidal gold and ELISA detect the result comparison of anti-RV IgG

[0047]

[0048] From the data in Table 5, the Youden index can be calculated as 534 / (534+34)+1562 / (1+1562)-1=0.94, indicating that the overall coincidence rate between the test results and the real situation of the samples is very high. From the data in Ta...

Embodiment 3

[0056] Embodiment 3: detect anti-herpes simplex virus (HV) IgM, IgG antibody

[0057] A colloidal gold chromatography strip for detecting anti-herpes simplex virus (HV) IgM and IgG antibodies was prepared by a method similar to that of Example 1. The prepared colloidal gold chromatography strip was used to detect 326 anti-HV IgG positive sera and 1863 negative sera with clear clinical diagnosis, and compared with the results of anti-HV IgG detected by indirect ELISA reagent alone. The results are shown in Table 9 and Table 10.

[0058] The detection result of table 9 colloidal gold chromatography strip anti-HV IgG clinical serum

[0059]

[0060] Table 10 Colloidal gold and ELISA detect the result comparison of anti-HV IgG

[0061]

[0062] From the data in Table 9, the Youden index can be calculated as 304 / (304+22)+1862 / (1+1862)-1=0.93, indicating that the overall coincidence rate between the test results and the real situation of the samples is very high. From the d...

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Abstract

The invention provides a double capture method to detect colloidal gold chromatography strip of the IgM, IgG antibody and its preparation technology. The chromatography strip takes the colloid gold as the marking matter, one end of the PVC backing mutually builds the sample pad, the compound pad, the nitryl cellulose membrane, another end pastes the absorb pad; the states compound pad is the glass cellulose membrane which coated with the antigen - colloid gold compound, which characteristic lies in: it contains three peridium lines on the states nitryl cellulose membrane, separately peridium the anti- IgG gamma chain antibody, the anti- IgM mu chain antibody, and the antibody which corresponds to antigen. The invention chromatography strip uses the double capture method to detect the IgM antibody, IgG antibody, through once operation detects the specificity IgM, IgG antibody, which simplified the operating process, and the examination result of which conforms to the actual examinationwork need.

Description

technical field [0001] The invention relates to a chromatographic strip for detecting specific IgM and IgG antibodies by means of an immunoassay method and a preparation technology thereof, in particular to an immunochromatographic reaction for specific IgM and IgG antibodies by means of colloidal gold labeling for color development. A chromatographic strip for combined detection and a preparation method thereof. Background technique [0002] Immunoglobulin M (Immunoglobulin M, IgM) and Immunoglobulin G (Immunoglobulin G, IgG) are the two most important antibodies in humans or animals. After an antigen (bacteria or virus, etc.) enters the body, the first immunoglobulin that appears is IgM antibody, and then IgG antibody appears. Although IgA antibody may also appear in some cases, because its clinical significance is basically the same as that of IgG, and the IgA content is very low, it has little practical significance in clinical testing, so it is generally not distinguis...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/558
Inventor 陈廷友王健王志新张秀杰
Owner 北京英诺特生物技术股份有限公司
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