Edible mushroom liquid strain solidifying processing method
A liquid strain, solidification treatment technology, applied in the direction of fungi, etc., can solve the problems of loss of vitality, high cost, intolerance to storage, etc., to achieve the effect of avoiding strain degradation, reducing the number of passages, and reducing production costs
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[0019] The solidification treatment method of the edible fungus liquid strain of the present invention is as follows:
[0020] (1) The test tube preserved species are expanded and activated, and the preserved female species (Pleurotus ostreatus, Shiitake, Agaricus bisporus, Flammulina velutipes, Coprinus comatus, Ganoderma lucidum, Pleurotus eryngii, Fungus, Shimeji mushroom, Pleurotus eryngii, Chaxing mushroom) Any one) is transferred to the slope of the test tube and incubated at room temperature;
[0021] (2) Making shake flask liquid strain
[0022] Prepare the appropriate liquid culture medium, fill the liquid culture medium with 100ml / 250ml or 150ml / 500ml in the corresponding conical flask, sterilize for 30 minutes at 121°C and 1 kg / cm² pressure, and insert it after cooling. Strains about square centimeters, cultured on a shaker at 25℃, 150r / min;
[0023] (3) Deep culture in fermenter
[0024] Prepare liquid culture medium according to 75% of the total capacity of the fermen...
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