Edible mushroom liquid strain solidifying processing method

A liquid strain, solidification treatment technology, applied in the direction of fungi, etc., can solve the problems of loss of vitality, high cost, intolerance to storage, etc., to achieve the effect of avoiding strain degradation, reducing the number of passages, and reducing production costs

Inactive Publication Date: 2007-12-12
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although solid strains have the advantages of being easy to operate, requiring simple equipment, low cost, and being convenient for transportation, they are generally accepted by mushroom growers and food (medicine) fungus cultivation plants, but there are still some disadvantages: long cultivation time and many generations of strains , not resistant to preservation, some strains will have the shortcomings of fruiting bodies during the cultivation process
Usually, liquid strains have the advantages of short germination time, higher strain quality and lower production cost, but have the following disadvantages: (1) not resistant to storage, once the liquid strains are fermented, they should be used as soon as possible. Otherwise, if the time is longer, the bacteria will age rapidly and lose their vitality
The traditional production method of solid strains is labor-intensive and time-consuming. Take the species with faster mycelium growth in edible (medicinal) fungi, it takes about 60 days from the production of the test tube mother species to the cultivation of the cultivated species. In addition, not only It is necessary to cool down in summer and heat up in winter to meet the optimum temperature for mycelial growth, and it takes a lot of manpower and material resources to sort and turn bags, which obviously does not meet the requirements of large-scale production for strains.
However, the existing production methods of liquid strains require a relatively high technical level of professionals, which makes it only used in large scientific research institutes, and it is difficult to popularize for decentralized mushroom farmers.

Method used

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Experimental program
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Effect test

Embodiment Construction

[0019] The solidification treatment method of the edible fungus liquid strain of the present invention is as follows:

[0020] (1) The test tube preserved species are expanded and activated, and the preserved female species (Pleurotus ostreatus, Shiitake, Agaricus bisporus, Flammulina velutipes, Coprinus comatus, Ganoderma lucidum, Pleurotus eryngii, Fungus, Shimeji mushroom, Pleurotus eryngii, Chaxing mushroom) Any one) is transferred to the slope of the test tube and incubated at room temperature;

[0021] (2) Making shake flask liquid strain

[0022] Prepare the appropriate liquid culture medium, fill the liquid culture medium with 100ml / 250ml or 150ml / 500ml in the corresponding conical flask, sterilize for 30 minutes at 121°C and 1 kg / cm² pressure, and insert it after cooling. Strains about square centimeters, cultured on a shaker at 25℃, 150r / min;

[0023] (3) Deep culture in fermenter

[0024] Prepare liquid culture medium according to 75% of the total capacity of the fermen...

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Abstract

The invention relates to a method for solidifying liquid bacterial for edible mushroom. It comprises following steps: (1) activating preserved parent strain through enlarge test: inoculating stored parent bacterial to inclined surface of test tube, culture at proper temperature; (2) producing shake flask liquid bacterial; (3) deep culturing fermentation tank; (4) carrier physical treatment; (5) solidifying liquid bacteria. The invention produces solid bacteria at opening environment, which overcomes regular applicatio and limit for liquid bacteria; saves culture process, and realizes convenient usage of liquid bacteria. The invention plays a important role in promoting edible mushroom conversion from traditional mode to industrial mode.

Description

Technical field [0001] The invention relates to a method for treating edible fungi strains, in particular to a solidification treatment method for edible fungi liquid strains. Background technique [0002] Edible (medicinal) fungus is the only non-photosynthetic plant food (medicine) that is consumed in large quantities worldwide and the market trend has been promising. It is rich in natural protein, amino acids, vitamins, and a variety of mineral elements. Its pharmacological effects are favored by the world. Because its production relies on agricultural and forestry organic waste materials, these waste organic materials can be decomposed and converted into high-quality protein. This not only eliminates environmental pollution, creates a good ecological environment, realizes zero emission (zero emmision), but also provides food (medicine) products that humans need, creating considerable economic and social benefits, so the food (medicine) fungus industry has been able to achieve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14
Inventor 邱立友王兰青王淑敏戚元成高玉千梁振普申进文陈钢刘全军
Owner HENAN AGRICULTURAL UNIVERSITY
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