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Phosphoric acid enol type pyruvate carboxylase and coding gene thereof

A technology of pyruvate carboxylase coding and pyruvate carboxylase, which is applied in genetic engineering, plant gene improvement, lyase, etc., can solve the problem of not being able to determine the exact function of bacterial PEPC

Inactive Publication Date: 2008-01-09
TSINGHUA UNIV
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  • Description
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  • Application Information

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Problems solved by technology

In addition, bacterial PEPC has also been found in rice and soybean, but so far, people have not been able to determine the exact function of bacterial PEPC in plants

Method used

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  • Phosphoric acid enol type pyruvate carboxylase and coding gene thereof
  • Phosphoric acid enol type pyruvate carboxylase and coding gene thereof
  • Phosphoric acid enol type pyruvate carboxylase and coding gene thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1, the acquisition of GhPEPC2 and its expression characteristics

[0047] cotton material

[0048] The plant material used in this experiment is an excellent cotton variety cultivated in my country: Zhongmian 35 (Gossypium hirsutum cv. zhongmian 35). The seeds of Zhongmian 35 were sown in small pots and grown in a greenhouse (25° C., 16 hours light / day). After about two weeks of growth, the roots, stems, cotyledons and true leaves of the seedlings were taken, quickly frozen in liquid nitrogen, and then stored at -80°C for future use. The flowers, seeds, embryos and fibers (5 days) of adult Cotton 35 plants grown in the greenhouse were quickly frozen in liquid nitrogen and stored at -80°C for later use.

[0049] 1. Cloning of GhPEPC2

[0050] After investigation, some cloned PEPCs were found, one of which was from cotton (GhPEPC1). According to the reported DNA sequence of plant PEPC, PEPC from different sources were compared to find the conserved region in P...

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Abstract

A phosphoenol-pyruvate carboxylase and its encoding gene are disclosed. It consists of (a) or (b) protein, (a) protein is made of amino-acid residue sequence in sequence 2; (b) protein is derived by (a) and substituted and / or lost and / or added by one or several amino acid in sequence 3 and has phosphoenol-pyruvate carboxylase activity. It can be used to increase fatty content in plant.

Description

technical field [0001] The invention relates to a phosphoenol pyruvate carboxylase and its coding gene. Background technique [0002] Phosphoenolpyruvate carboxylase (phosphoenopyruvate carboxylase; EC.4.1.1.31) can convert HCO 3 - and phosphoenol pyruvate (phosphoenopyruvate, PEP) irreversibly converted to oxaloacetate (oxaloacetate, OAA) and inorganic phosphorus (Utter, M.F, and Kolenbrander, H.M.1972.Formation of oxaloactetate by CO2 fixation on phosphoenolpyruvate.In TheEnzymes( Boyer, P.D., ed.). New York: Academic Press, pp.117-136) (Fig. 1), by Bandurski and Greiner (Bandurski RS, Greiner CM.1953.The enzymaticsynthesis of oxalacetate from phosphoenolpyruvate and carbon dioxide.J .Biol.Chem.204,781-786) first found in spinach leaves. It exists in the form of homotetramers in vivo, and the size of the monomer is 100-110 kDa (O'Leary M.1982.Phosphoenolpyruvatecarboxylase: an enzymologist's view.Annu.Rev.Plant Physiol.33:297-315 ). In 1984, PEPC was first cloned from...

Claims

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Application Information

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IPC IPC(8): C12N9/88C12N15/60C12N5/10
Inventor 刘进元乔志新
Owner TSINGHUA UNIV
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