Fluorescence photometry for immune affinity column of aflatoxin in paddy

A technology of aflatoxin and fluorescence photometry, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of long time, high labor intensity, poor sensitivity, etc., and achieve the goal of reducing detection cost, reducing usage, and high precision Effect

Inactive Publication Date: 2008-01-23
JING BRAND
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] (1) In the process of operation, it is necessary to use highly toxic aflatoxin as a calibration standard, which is harmful to the safety of operators
[0004] (2) The operation process is cumbersome, complicated, time-consuming and labor-intensive
[0005] (3) The instruments and equipment are expensive, bulky, and complicated to operate, making it difficult to achieve rapid on-site analysis
[0006] (4) The sensitivity is poor, and the repeatability is difficult to obtain satisfactory results
[0029] 2.6 Glass test tube: 12mm in diameter, 75mm in diameter, no fluorescence

Method used

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  • Fluorescence photometry for immune affinity column of aflatoxin in paddy
  • Fluorescence photometry for immune affinity column of aflatoxin in paddy

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Embodiment Construction

[0074] specific implementation

[0075] Specific examples of the present invention are as follows. The present invention is through test and research, and the method of this prior art has been improved and optimized, and the detection method of aflatoxin in the improved paddy is carried out according to the following steps:

[0076] 1 Reagents and solutions

[0077] 1.1 water: the water mentioned in the method of the embodiment of the present invention all refers to redistilled water

[0078] 1.2 Methanol: chromatographically pure

[0079] 1.3 60-80% methanol-water solution: Take 60mL of methanol and add 40mL of water, and so on, to prepare a 70% or methanol solution.

[0080] 1.4 Methanol-water (2+8): Take 20mL of methanol and add 80mL of water

[0081] 1.5 Sodium chloride (Nacl): analytically pure

[0082] 1.6 PBS buffer solution: 8.0g sodium chloride, 1.2g disodium hydrogen phosphate, 0.2g potassium dihydrogen phosphate, 0.2g potassium chloride, dissolve with 990mL pur...

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Abstract

The invention relates to a method for determining the fluorescent luminosity for the immunity affinity column of aflatoxicosis in rice, which comprises such procedures as extracting, cleaning, determining and calculating, etc.; and is characterized in that: the extracting procedure is to fetch 10.0g sample, add 2.0-2.5g NaCl and 60-80% methanol-water solution 50.omL, and extract by ultrasonic for 10-20 min; in the cleaning procedure, when cleaning the affinity column, first the affinity column is showered by a tween -20 / PBS solution, then by a methanol: water =2:8 solution, finally by water. The invention has the advantages that the recovery rate is more near to the actual value, the accuracy is higher, the reagent is reduced, the inspection cost is reduced, the environmental pollution is reduced, and the calculation is convenient.

Description

technical field [0001] The invention belongs to the technical field of food safety detection. Background technique [0002] Methods for the determination of aflatoxins in grains include thin-layer chromatography, high performance liquid chromatography, enzyme-linked immunosorbent assay, mass spectrometry, and radioimmunoassay. These methods more or less all have following deficiencies: [0003] (1) During the operation, it is necessary to use highly toxic aflatoxin as a calibration standard, which is harmful to the safety of operators. [0004] (2) The operation process is cumbersome, complicated, time-consuming and labor-intensive. [0005] (3) The instruments and equipment are expensive, bulky, and complicated to operate, making it difficult to achieve rapid on-site analysis. [0006] (4) The sensitivity is poor, and the repeatability is difficult to obtain satisfactory results. [0007] At present, the "aflatoxin immunoaffinity column-fluorescence photometer method" c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/06G01N30/02G01N33/577
Inventor 汪咏曾张继斌
Owner JING BRAND
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