Intestinal flora isolated culturing device

A technology of intestinal flora, in vitro culture, applied in the simulated intestinal, continuous flow of the intestinal flora in vitro culture device, to study and observe the impact of foreign drugs or food on the intestinal flora, and can solve the problem of temperature sensitivity The problems such as adverse effects on the growth of micro-organisms, high price, uneven stirring of the fermentation contents, etc., achieve the effect that the solution volume remains stable and the stirring is uniform and uniform.

Inactive Publication Date: 2008-01-30
SOUTH CHINA AGRI UNIV
View PDF0 Cites 22 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The key part of the experimental device is the fermenter, which has automatic heating, automatic stirring, automatic flow rate control, etc., but the equipment is not only expensive, but also has some defects in

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Intestinal flora isolated culturing device

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] In order to achieve the purpose of the invention, the structure of an intestinal flora in vitro culture device of the present invention is as shown in Figure 1. It includes a temperature control system for a fermenter, an automatic delivery of fresh medium and an output waste liquid system, and an intestinal flora growth facility. An environmental protection system is required. The first technical scheme adopted is that the temperature control system of the designed fermenter is composed of a fermenter 1 with a water bath jacket 16, a miniature submersible pump 6 and a constant temperature water bath 7. The fermenter 1 is a double-layer outer wall glass culture bottle with a water bath jacket. The upper end of the outer wall of the water bath jacket 16 of the fermenter 1 is provided with a water outlet 19 and the lower end is provided with a water inlet 18. They are connected with the micro submersible pump 6 and the constant temperature water bath respectively. 7 are c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention relates to an in vitro culture device, which can simulate intestinal flora for intestinal and continuous flow, comprising a temperature control system of a fermentation tank, an automatic fresh culture medium feeding and a waste liquid output system and a safeguard system for an environment required by the growth of the intestinal flora; the fermentation tank that is connected with a constant temperature water bath cauldron and a micro submersible and is provided with a water bath clamp ferrule is designed; the culture medium can continuously flow into while the waste liquid can continuously flow out the system through the connection of a peristaltic pump; an anaerobic environment, pH scope and nutrient conditions are reached by inputting nitrogen and a pH controller. The human intestinal flora culture device successfully constructed in the invention is characterized by low cost, easy use and simple operation. Besides, the human intestinal flora can grow stably upon inspection and the comparability of the structure of the intestinal flora is up to 80 percent or more compared with the intestinal flora before the culture. Therefore, the invention is applicable to the safety evaluation and research of the influence to the human intestinal flora caused by antibacterial residues, and the invention is also applicable to the function evaluation of nutrient substances and function and safety evaluation and research of medicines and foods.

Description

technical field [0001] The invention relates to an in vitro culture device for bacterial flora, in particular to an in vitro culture device for intestinal flora capable of simulating the intestinal tract and continuously flowing, and is especially suitable for studying and observing the influence of foreign medicine or food on the intestinal flora. Background technique [0002] Gut flora is a balanced ecosystem that plays a very important role in maintaining the health of humans and animals. While inhibiting the growth of pathogenic bacteria, antibacterial drugs can also affect the normal intestinal flora, disrupt its balance, and cause superinfection. In recent years, studies have found that antibacterial drugs remaining in animal foods can also have adverse effects on the intestinal flora of the human body, and some functional foods such as probiotics can regulate the intestinal flora of humans and animals. Therefore, the safety or functional evaluation of drugs and funct...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C12M3/00C12M1/00
CPCC12M21/06C12M23/36C12M27/02C12M41/22C12M41/26
Inventor 陈杖榴刘健华高延玲曾振灵杨桂香刘雅红黄显会陈建新潘广方
Owner SOUTH CHINA AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap