Food for preventing life style-related diseases
A technology of living habits and food, which is applied in the field of food or medicine and quasi-drugs, and can solve problems such as protein deficiency
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preparation example 1
[0060] Preparation example 1: Preparation of mung bean protein decomposition product-1
[0061] The by-product of the vermicelli manufacturing process, that is, the dried product of the discharged liquid containing mung bean protein is pulverized with a pulverizer, and tap water is added for dispersion so that the crude protein content is 6% by mass. The pH was adjusted to 3 with hydrochloric acid, and 3000 units of acid protease (manufactured by Napsin, Nagakushintec) was added per 1 g of crude protein, and the reaction was carried out at 40°C for 16 hours under stirring. After the reaction is over, heat at 80-85°C for 20 minutes to inactivate the enzyme. The enzyme reaction solution was centrifuged, sodium hydroxide was added to adjust the supernatant to pH 8, and then activated carbon treatment, filtration, and filtration sterilization were performed, and then freeze-dried to obtain a mung bean protein decomposition product. According to the above procedure, the molecular wei...
preparation example 2
[0062] Preparation example 2: Preparation of mung bean protein decomposition product-2
[0063] According to the same method as Preparation Example 1, a dispersion liquid with a crude protein content of 6% by mass was prepared. After adjusting the pH to 7 with sodium hydroxide, add 10,000 units of alkaline protease per 1g of crude protein (ビオプラ-ゼSP15-FG (trade name), manufactured by ナガセケムテツクス company), stirring slowly at the same time React at ℃ for 16 hours. After the reaction, heat at 80-85°C for 20 minutes to inactivate the enzyme. The enzyme reaction solution was centrifuged, and the supernatant was freeze-dried to obtain a mung bean protein decomposition product. According to the above procedure, the molecular weight distribution of this decomposition product was measured. The ratio of dipeptides to tripeptides in the total peptides of this degradation product was 61.1%.
preparation example 3
[0064] Preparation Example 3: Preparation of feed containing mung bean protein decomposition product
[0065] As the rat test feed used in Example 1 below, the mung bean protein decomposed product obtained in the above Preparation Example 1 was mixed as crude protein into the powder feed of CRF-1 (Oriental Yeast Co., Ltd.) and each contained 0.25% W / w, 1.25% w / w and 2.50% w / w crude protein were prepared into granules with a tablet machine.
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