Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Application of realgar microorganism extracting liquid in preparing anti-tumor medicine

A technology of microbial leaching and leaching solution, which can be used in antitumor drugs, drug combinations, drug delivery and other directions, and can solve the problems of gastrointestinal irritation, difficulty in taking, and difficulty in popularizing and using

Inactive Publication Date: 2008-02-13
李红玉 +1
View PDF0 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this kind of treatment can still only be used as a suspension, and there are still problems such as gastrointestinal irritation caused by oral administration, and difficulty in taking it. In addition, the production of nano-realgar requires large-scale equipment and advanced technology, and it is still difficult to popularize it. [7]

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Preparation of realgar microbial extract

[0027] Treat realgar according to the mineral drug bacterial treatment method disclosed in Chinese invention patent 2006102000675, the implementation plan is as follows: Weigh 0.5g realgar powder over 200 mesh in a sterilized 250mL Erlenmeyer flask, add sterilized 9K iron-free liquid for culture Base 90mL, adjust the pH to 1.75 with sulfuric acid, after the pH is stable, inoculate a volume fraction of 20% Thiobacillus ferrooxidans and a final concentration of 1.0g / L Fe 2+. After weighing the mass, shake the flask for 30 days at 30°C with a shaking speed of 135r / min. During the experiment, 1:1 (volume ratio) sulfuric acid was used to adjust the pH value, and distilled water was used to supplement the evaporated water. Shake the flask for leaching and stop after 30 days, collect the supernatant by suction filtration under reduced pressure, adjust the pH of the leachate to about 7, and pass through a 0.22 micron filter membrane ...

Embodiment 2

[0029] Inhibitory effect of different concentrations of realgar microbial leachate on the growth of liver cancer cell line HepG-2

[0030] Cell culture: use RPMI1640 medium, add 10% bovine serum, penicillin 100U / ml, streptomycin 100U / ml, place at 37°C, 5% CO 2 cultivated under conditions. Change the culture medium every 2-3 days.

[0031] MTT assay: the cells were approximately 5 × 10 4 Cells / ml were seeded on a 96-well plate at 100 microliters per well and cultured overnight. At 24 hours, 48 ​​hours and 72 hours respectively, the realgar microbial extract was added according to the preset concentration gradient, and each gradient was repeated at least three times. After continuing to cultivate for 24 hours, add 20 microliters of 5 mg / ml MTT to each well, continue to incubate at 37°C for 4 hours, quickly discard the supernatant by flipping the plate, add 150 microliters of DMSO to each well, On-device detection, calculate the half inhibitory concentration IC according to t...

Embodiment 3

[0035] Growth inhibitory effect of realgar microbial extract on gastric cancer cell line MGC-803

[0036] Cell culture: use RPMI1640 medium, add 10% bovine serum, penicillin 100U / ml, streptomycin 100U / ml, place at 37°C, 5% CO 2 cultivated under conditions. Change the culture medium every 2-3 days.

[0037] MTT test: the cells were approximately 10 5 Cells / ml were inoculated on a 96-well plate, 100 microliters per well, and cultured overnight. At 24 hours, 48 ​​hours and 72 hours respectively, the realgar microbial extract was added according to the preset concentration gradient, and each gradient was repeated at least three times. After continuing to cultivate for 24 hours, add 20 microliters of 5 mg / ml MTT to each well, continue to incubate at 37°C for 4 hours, quickly discard the supernatant by flipping the plate, add 150 μg of DMSO to each well, and place in a microplate reader. On detection, calculate the half inhibitory concentration IC according to the measured OD va...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to the application of the realgar microorganism leaching solution in anti tumor medicine preparation. The realgar microorganism leaching solution is prepared according to the exposed microorganism treating method in patent 2006102000675. The realgar leaching solution in the present invention has a profound inhabiting effect in a plurality of cancer disease comprising cancer of the liver, gastric cancer, leukemia and lung cancer. In addition, the application quantity is in level of microgram. Furthermore, because the realgar microorganism leaching solution is a dissoluble medicine, therefore the medicine can be applied respectively or applied in combination with other drugs, or with pharmaceutical acceptable excipient. The medicine is easy to be produced as various kinds of preparations, which comprises guttate pills, aerosol, vinum, syrups, oral application liquid, injection and injectable powder.

Description

technical field [0001] The invention relates to the application of realgar microbiological leachate in the preparation of antitumor drugs. Background technique [0002] Realgar mainly contains arsenic tetrasulfide (As 4 S 4 ), some people also think that its main content is arsenic disulfide (As 2 S 2 ), also known as gold stone, is a common mineral traditional Chinese medicine. Since "Shen Nong's Materia Medica", it has a medical history of more than 2,000 years in China. Realgar has the effects of detoxifying and killing insects, drying dampness and eliminating phlegm, and cutting off malaria. [1] , modern medicine believes that realgar has anti-tumor effect, and has been successfully used in the treatment of chronic and acute myeloid leukemia in clinic [2，3，4] , so more and more scholars at home and abroad have received extensive attention and become a hot spot in mineral Chinese medicine research. [0003] Mineral medicine is a cultural treasure of the Chinese nat...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/36A61K9/08A61K9/02A61K9/20A61K9/16A61K9/50A61K9/12A61K9/10A61K9/14A61P35/00A61P35/02
Inventor 李红玉张旭谢亲建王欣
Owner 李红玉
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com