Method for measuring fructosamine NBT in blood serum

A determination method and fructosamine technology are applied in the field of NBT determination of fructosamine in serum to achieve the effects of solving the problem of precipitation, eliminating lipid turbidity and reducing interference

Inactive Publication Date: 2008-03-05
NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] 3. The precipitation of NBT in a single reagent alkaline environment, etc.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] The method for determination of fructosamine NBT in serum, its specific steps are:

[0037] 1. Provide standard solution, R1, R2 reagents and samples to be tested;

[0038] The preparation method of the standard solution is as follows: 1. Mix serum from healthy people, freeze and thaw several times until the upper layer is clear, and take the clear upper layer (the lower layer is discarded). After filtration through a filter with a 0.15-0.30 μm membrane, it is used as the matrix of the standard solution. First measure the albumin content, then add bovine serum albumin to a concentration of about 4.0% by weight, and finally add 0.05% by weight of biological preservatives (such as methyl-isothiazoline PC-300, etc.), mix well ,stand-by;

[0039] ②Add disodium ethylenediaminetetraacetate (EDTA-Na2) 2g / L and lactose 30g / L to the above purified serum, shake well, and set aside;

[0040] 3. Measure the fructosamine concentration in the above serum, then add the fructosamine...

Embodiment 2

[0059] The method for determination of fructosamine NBT in serum, its specific steps are:

[0060] 1. Provide standard solution, R1, R2 reagents and samples to be tested;

[0061] The preparation method of the standard solution is as follows: 1. Mix serum from healthy people, freeze and thaw several times until the upper layer is clear, and take the clear upper layer (the lower layer is discarded). After filtration through a 0.15-0.30um membrane filter, it is used as the matrix of the standard solution. First measure its albumin content, then add bovine serum albumin to a concentration of about 4.0% by weight, and finally add 0.1% by weight of biological preservatives (such as methyl-isothiazoline PC-300, etc.) uniform, ready to use;

[0062] ②Add disodium ethylenediaminetetraacetate (EDTA-Na2) 2g / L and lactose 30g / L to the above purified serum, shake well, and set aside;

[0063] 3. Measure the fructosamine concentration in the above serum, then add the fructosamine dry po...

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PUM

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Abstract

The method comprises: its standard solution uses the healthy human blood serum as base substance; the fructosamine measuring reagent box uses a combination of dual reagents R1 and R2; said R1 comprises NBT reaction component, pH is neutrality; said R2 is alkalinity potassium carbonate buffer solution; the ratio of R1,P2 to the sample under test is 40-50: 8-12: 3.

Description

technical field [0001] The invention relates to a method for measuring fructosamine NBT in serum. Background technique [0002] Glycated serum protein (Glucosylated Serum Protein, GSP) is the product of slow non-enzymatic glycation reaction between various proteins in human serum and glucose. The main one is the glycation of albumin, so GSP can also be called glycated albumin. Since the half-life of albumin in the human body is 17 days, the determination of GSP can reflect the average blood sugar level in the 2 to 3 weeks before the measurement, and it is a good indicator for diabetic patients to control blood sugar. Therefore, the determination of GSP is of great significance to the diagnosis, prevention and treatment of diabetes, especially the prevention and treatment of various complications of diabetes. [0003] There are many methods for determining GSP, which are divided into two categories: chemical method and chromatography method; chemical method evaluates the le...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/68G01N21/17
Inventor 宋克征邹炳德
Owner NINGBO MEDICAL SYSTEM BIOTECHNOLOGY CO LTD
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