Method for extracting validamycin

A technology of Jinggangmycin and resin, which is applied in the extraction field of Jinggangmycin, can solve the problems of not meeting the quality requirements of Jinggangmycin products, failing to meet the needs of downstream product development, and being unable to open the international market, etc., achieving low cost and high adsorption capacity. Rapid, easy reprocessing effect

Active Publication Date: 2008-03-12
ZHEJIANG UNIV OF TECH +2
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The color is dark, the content of active component Jinggangmycin A is low, and the product quality is low, which cannot meet the needs of downstream product development, nor meet the quality requirements of Jinggangmycin product export, and has been unable to open the international market for a long time
[0009] Zheng Yuguo et al. (Pesticides, 1996, 35 (11): 9~10) reported the use of gel ion exchange resins to extract and separate Jinggangmycin in the fermentation broth. After the fermentation broth was pretreated, it was first removed by strong basic anion exchange resin Impurities, and then use strong acid resin gel resin to adsorb Jinggangmycin to obtain high-purity Jinggangmycin products, but there are problems such as long process flow, low adsorption capacity, long desorption time, large wastewater discharge, and high cost.

Method used

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  • Method for extracting validamycin
  • Method for extracting validamycin

Examples

Experimental program
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Effect test

Embodiment 1

[0044] Fermentation of Jinggangmycin: the strain used is Streptomyces hygroscopicus var. Jinggangensis. Fermentation medium composition (g / l): 90g corn flour, 40g soybean flour, 5g yeast extract, 0.1g K 2 HPO 4 , fermented in a stirred fermenter at a fermentation temperature of 39° C., an aeration rate of 1.0 vvm, and a fermentation time of 45 hours to obtain Jinggangmycin fermentation broth. After analysis, the concentration of Jinggangmycin A in the fermentation broth was 18mg / ml.

[0045] Take 10L of fermented fermentation broth and heat it to 120°C, keep it for 15 minutes, and filter. The filtrate was passed through MN-500 super large network cation adsorption resin (HYPERSOL-MACRONET TM , Piao Laite China Company) for ion exchange and adsorption, the flow rate is: 1.0m 3 / m 3 Resin·h, until the resin is adsorbed and saturated (detected by high performance liquid chromatography while eluting, and stop adding the filtrate when the saturation point is reached). Then wa...

Embodiment 2

[0048] The fermentation liquid preparation method of Jinggangmycin is the same as embodiment 1

[0049] Take 10L of fermented fermentation broth and heat it to 100°C, keep it for 45 minutes, and filter. The filtrate was passed through MN-500 super large network cation adsorption resin (HYPERSOL-MACRONET TM , Piao Laite China Company) for ion exchange and adsorption, the flow rate is: 1.0m 3 / m 3 Resin·h, until the resin is adsorbed and saturated (detected by high performance liquid chromatography while eluting, and stop adding the filtrate when the saturation point is reached). Then wash the resin with deionized water twice the volume of the resin, and the washing speed is 10m 3 / m 3 Resin h. After washing, use the aqueous ammonia solution of 0.5mol / L as eluent, and elute to the end point according to conventional methods (use high-performance liquid chromatography to detect while eluting, and stop adding eluent when reaching the end point), and the elution rate is 1.0m ...

Embodiment 3

[0051] The fermentation of Jinggangmycin is the same as in Example 1

[0052] Take 10L of fermented fermentation broth and heat it to 120°C, keep it for 15 minutes, and filter. The filtrate passes through C104E resin for ion exchange and adsorption, the flow rate is: 1.0m 3 / m 3 Resin·h, until the resin is adsorbed and saturated (detected by high performance liquid chromatography while eluting, and stop adding the filtrate when the saturation point is reached). Then wash the resin with deionized water twice the volume of the resin, and the washing speed is 3m 3 / m 3 Resin h. After washing, use the aqueous ammonia solution of 1.5mol / L as the eluent, and elute to the end point according to conventional methods (use high-performance liquid chromatography to detect while eluting, and stop adding eluent when reaching the end point), and the elution rate is 1.0m 3 / m 3 Resin h. The eluate obtained was collected, evaporated and concentrated under reduced pressure, so that the...

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Abstract

The present invention relates to the extraction method of Jinggang adriamycin. In the method, Jinggang adriamycin fermentation liquid is heated to the temperature between 100 DEG C and 120 DEG C for 5 minutes to 60 minutes. Then the Jinggang adriamycin fermentation liquid is filtered. The filtrate experiences the ion exchange and adsorption through the extra-large positive ion resin adsorption net. And then 0.1-1.5mol per L ammonia solution is used as eluant which cleans the extra-large positive ion resin adsorption net of Jinggang adriamycin. The eluant is collected and vaporized under low pressure to get rid of ammonia gas. After that, crud Jinggang adriamycin A product is achieved, which is further processed to generate Jinggang adriamycin. The method is of short technological process, large absorption quantity, short desorption time, little wastewater emission and low cost.

Description

technical field [0001] The invention relates to a method for extracting Jinggangmycin, in particular to a method for extracting Jinggangmycin with super large net cation adsorption resin. Background technique [0002] Jinggangmycin is an agricultural antibiotic independently developed and produced by academician Shen Yinchu and others in my country. It is produced by the metabolism of Streptomyces hygroscopicus var. With the advantages of long growth, no resistance, and stable vitality, after years of development, it has become a very important variety of biopesticides in my country. It has made a major contribution to the high and stable yield of rice in my country, and has a very important role in agricultural production and even the national economy. It has become one of the antibiotics with the largest output and the highest fermentation unit in my country. The fermentation titer of Jinggangmycin A has reached above 30,000 μg / ml, and it has developed into an important bio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H15/22A01N63/02A01P3/00
Inventor 郑裕国沈寅初薛亚平陈小龙
Owner ZHEJIANG UNIV OF TECH
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