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Reducibility coenzyme composite stabilizer

A compound stabilizer and stabilizer technology, applied in the field of diagnostic reagents, can solve the problems of affecting the performance of reagents, the impact of clinical disease diagnosis results, and the instability of reagents.

Inactive Publication Date: 2008-03-12
SHANGHAI FOSUN PHARMA (GROUP) CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Because these reagents are very unstable, which affects the performance of the above reagents, it will also have a significant impact on the diagnostic results of clinical diseases
At present, there is still a lack of an effective method at home and abroad to protect the stability of reduced nicotinamide purine dinucleotide (NADH) and reduced nicotinamide purine dinucleotide phosphate (NADPH)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0031] CAPSO: 5g

[0032] Glycerin: 20g

[0033] Ethylene glycol 50g

[0034] Sodium chloride 9g

[0035] EGTA0.42g

[0036] NaN 3 1g

[0037] Weigh the above substances and add them into 0.8L deionized water in turn, adjust the pH value to 9.5 after the dissolution is complete, and then dilute to 1L for use.

[0038] Weigh 0.3g NADH and dissolve in the above 1L mixed solution (No. I solution) and measure 6 bottles of 50mL NADH mixed solution respectively (referred to as A, B, C, D, E, F in order)

[0039] Bottle A: put in a refrigerator at 4°C

[0040] Bottle B: put in an oven at 37°C for 1 day

[0041] Bottle C: put in an oven at 37°C for 2 days

[0042] Bottle D: put in an oven at 37°C for 3 days

[0043] Bottle E: put in an oven at 37°C for 4 days

[0044] F bottle: put in an oven at 37°C for 5 days

[0045] At 340nm, we use a spectrophotometer to detect

[0046] A

[0047] Visible, in accelerated experiment, NADH drops very little in the stabili...

example 2

[0061] AMPSO 100g

[0062] EDTA 5g

[0063] KCl 50g

[0064] proclin300 0.1g

[0065] Benzene glycol 200g

[0066] BSA 100mg

[0067] Weigh the above substances and add them into 0.8L deionized water in turn, adjust the pH value to 9.5 after the dissolution is complete, and then dilute to 1L for use.

[0068] Weigh 0.3g NADPH and dissolve it in the above 1L mixture, measure 6 bottles of 50mL NADPH mixture respectively (referred to as A, B, C, D, E, F in order)

[0069] Bottle A: put in a refrigerator at 4°C

[0070] Bottle B: put in an oven at 37°C for 1 day

[0071] Bottle C: put in an oven at 37°C for 2 days

[0072] Bottle D: put in an oven at 37°C for 3 days

[0073] Bottle E: put in an oven at 37°C for 4 days

[0074] F bottle: put in an oven at 37°C for 5 days

[0075] At 340nm, we use a spectrophotometer to detect

[0076] A

[0077] It can be seen that the stabilizer of the present invention has a significant effect on the stability of NADPH. ...

example 3

[0079] AMPSO 75g

[0080] CAPSO 7.5g

[0081] Dithiothreitol 30g

[0082] Propanol 25g

[0083] NA2SO4 20g

[0084] Gentazyme 1.0g

[0085] BSA 100mg

[0086]Weigh the above substances and add them into 0.8L deionized water in turn, adjust the pH value to 9.5 after the dissolution is complete, and then dilute to 1L for use.

[0087] Weigh 0.3g NADH and dissolve it in the above 1L mixed solution, and measure 6 bottles of 50mL NADH mixed solution respectively (referred to as A, B, C, D, E, F in order)

[0088] Bottle A: put in a refrigerator at 4°C

[0089] Bottle B: put in an oven at 37°C for 1 day

[0090] Bottle C: put in an oven at 37°C for 2 days

[0091] Bottle D: put in an oven at 37°C for 3 days

[0092] Bottle E: put in an oven at 37°C for 4 days

[0093] F bottle: put in an oven at 37°C for 5 days

[0094] At 340nm, we use a spectrophotometer to detect

[0095] A

[0096] It can be seen that the stabilizer of the present invention has obvious ...

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PUM

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Abstract

The present invention provides a reductive coenzyme composite stabilizing agent, which comprises buffering agent, stabilizing agent, organic solvent, chelator, anticorrosive agent and inorganic salt. The present invention has quite high stability for reductive nicotinamide adenine dinucleotide (NADH) and reductive Nicotinamide adenine dinucleotide 2'-phosphate reduced tetrasodium salt (NADPH).

Description

technical field [0001] The invention relates to a diagnostic reagent, in particular to a reducing coenzyme complex stabilizer. Background technique [0002] At present, there are many products using reduced nicotinamide purine dinucleotide (NADH) and reduced nicotinamide purine dinucleotide phosphate (NADPH) as reaction substrates in diagnostic reagents. Such as alanine aminotransferase (ALT), aspartate aminotransferase (AST,) total bile acid (TBA), α-hydroxybutyrate dehydrogenase (α-HBDH), adenosine deaminase (ADA) ), direct bilirubin (DBIL), etc. The purpose of using NADH and NADPH is as a reaction indicator (the reaction is converted from NADH / NADPH to NAD / NADP, and its absorbance at 340nm decreases obviously) or as an anti-interference agent. The above diagnostic items have extensive significance in clinical diagnosis, especially in the application of hepatobiliary diseases. However, reduced nicotinamide purine dinucleotide (NADH) and reduced nicotinamide purine dinuc...

Claims

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Application Information

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IPC IPC(8): G01N33/52C12Q1/00
Inventor 孙卫兵
Owner SHANGHAI FOSUN PHARMA (GROUP) CO LTD
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