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Method for inhibiting brown stain generation of cymbidium plant tissue culture

A plant tissue culture and tissue culture technology is applied in the field of inhibiting browning in the process of tissue culture of orchid plants, can solve problems such as inability to solve browning well, and achieves the effects of low cost, low use concentration and easy operation.

Inactive Publication Date: 2008-03-26
SOUTH CHINA NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] The purpose of the present invention is to provide a method for inhibiting browning in the tissue culture process of Orchid plants, aiming at the problem that the prior art cannot solve the browning well

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Phalaenopsis explants were soaked at room temperature for 5 minutes with 0.01% naphthaleneacetic acid;

[0018] (2) 0.001% TDZ was added to the medium, and the light intensity was 2000 lux.

[0019] It is proved by experiments that the browning incidence rate of the Phalaenopsis control leaves is 62.3%, and the browning incidence rate of the leaves treated by the method is 52.2%.

Embodiment 2

[0021] (1) Chunlan explants were soaked at room temperature for 10 minutes with 0.03% gibberellic acid;

[0022] (2) 0.002% 4-PU was added to the medium, and the light intensity was 3000 lux.

[0023] The experiment proved that the incidence of browning of leaves of Chunlan control was 60.3%, and the incidence of browning of leaves treated by this method was 45.2%.

Embodiment 3

[0025] (1) Phalaenopsis explants soaked for 15 minutes with 0.05% gibberellic acid;

[0026] (2) 0.003% 6-BA was added to the medium, and the light intensity was 3000 lux.

[0027] It is proved by experiments that the browning incidence rate of the Phalaenopsis control leaves is 58.0%, and the browning incidence rate of the leaves treated by the method is 23.0%, effectively reducing the tannin content.

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Abstract

The improved tissue culture process capable of inhibiting browning for cymbidium plant includes the following steps: 1. sterilizing the surface of explant and soaking in solution with growth regulating matter gibberellic acid and / or auxin matter in 0.01-0.1 % concentration at normal temperature for 5-30 min; and 2. inducing indefinite bud generation in the culture medium with 6-BA, 4-PU or TDZ in 0.001-0.01 % concentration and in the illumination intensity of 2000-3000 lx. The present invention is simple and high in browning inhibiting effect.

Description

(1) Technical field [0001] The invention relates to a method for inhibiting the browning of orchid plant tissue culture. (2) Background technology [0002] Plant tissue culture technology is becoming more and more perfect, and has fully demonstrated its superiority in scientific research and modern agricultural production. It plays a huge role in the detoxification and rapid propagation of agricultural and forestry crops, mutation induction, reformed crop cultivation, cell engineering and genetic engineering breeding, and germplasm preservation. It is a high-tech with broad development potential. The steps that the tissue culture method of Orchid plant generally comprises at present are: first blade or pseudobulb explant are sterilized (generally adopt 75% ethanol and 0.1-0.2% mercuric chloride surface disinfection), then induce explant to produce adventitious bud ( The surface-sterilized explants are cut into pieces, placed in MS, B5 or White solid or liquid medium added w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/04A01H4/00
Inventor 李玲谭汝芳许传俊
Owner SOUTH CHINA NORMAL UNIVERSITY
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