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Method for separating and purifying bi(5-formylfurfuryl)ether

A technology of formyl furfuryl and ether, which is applied in the fields of organic chemistry and plant cells, can solve the problems of less research on active ingredients and achieve high yields

Inactive Publication Date: 2008-03-26
由翠荣 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

There is currently little research on the active ingredients in cyclamen

Method used

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  • Method for separating and purifying bi(5-formylfurfuryl)ether
  • Method for separating and purifying bi(5-formylfurfuryl)ether
  • Method for separating and purifying bi(5-formylfurfuryl)ether

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Embodiment 1

[0035] Cyclamen sterile seedling bulbs or petioles are inoculated on the following medium: 1 / 2MS+6-BA2mg / l+KT0.2mg / l+2,4-D 0.5mg / l to induce intracellular callus tissue; the intracellular callus was continuously subcultured in the following liquid medium: 1 / 2MS+6-BA0.5mg / l+NAA0.5mg / l, shaking culture (shaking culture conditions: 25°C, 120r / min shaker culture for 20 days) to obtain extracellular culture medium (1600ml); the extracellular culture medium was concentrated under reduced pressure to a paste, and dried at 60°C to obtain 54g of extract powder, which was mixed with 100ml of 1N NH 2 SO 4 Acid hydrolysis for 1 hour to obtain an acid hydrolysis solution; filter the acid hydrolysis solution, extract the filtrate with chloroform 3 times, combine the chloroform extracts for later use; The extracts were combined to obtain a crude chloroform extract; the crude chloroform extract was concentrated to dryness and eluted with ether to obtain an ether soluble; the ether soluble w...

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Abstract

The present invention discloses process of culturing, separating and purifying bis(5-formoxyl furfuryl) ether, and belongs to the field of plant tissue culturing technology. Extracellular culture fluid of cyclamen is first obtained through a conventional plant tissue culturing process of cyclamen as the material, and then treated through acidolysis, filtering, extracting, concentrating, crystallizing, re-crystallizing and other steps to obtain purified bis(5-formoxyl furfuryl) ether. The process can obtain bis(5-formoxyl furfuryl) ether in about 130 mg from 1.6 liter of the culture liquid and has certain industrial application foreground. The product bis(5-formoxyl furfuryl) ether may be applied in preventing and treating tuberculosis, etc.

Description

technical field [0001] The present invention relates to a method for preparing ether, in particular to a method for separating and purifying bis(5-formyl furfuryl) ether from the extracellular liquid of cultured cyclamen callus by means of plant tissue culture , belongs to the field of plant tissue culture. Background technique [0002] Cyclamen persicum Mill, also known as "rabbit ear flower" and "rabbit flower", belongs to the genus Cyclamen of Primulaceae (primulaceae) and is a perennial herbaceous plant. Ears are available in red, white, dark red and other colors. Native to Greece and Syria. At present, it is generally cultivated in greenhouses in my country, and it is one of the important types of the northern flower industry. In addition to its ornamental value, cyclamen has a variety of biological activities. In Turkish folk prescriptions, it has the functions of anti-fertility, promoting uterine contraction, stimulating menstruation and promoting blood circulation...

Claims

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Application Information

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IPC IPC(8): C12N5/04C07D307/46
Inventor 由翠荣曲复宁龚雪琴
Owner 由翠荣