In vivo gene transduction device

Abstract

The invention discloses a vivo gene transduction method and the device thereof. The device consists of a bielectrode needle provide with double syringe tubes, a high-voltage alternating current sine wave signal generator, a high-voltage direct current signal generator, and a microcomputer controlled chopping module. The invention adopts the bielectrode needle of the double syringe tubes to inject DNA into muscular tissues, and simultaneously applies excitation electric fields with specific parameters to the muscular tissues where the DNA is positioned, thereby realizing that the excitation electric field intensity of the original normal electrode device applied to the muscular tissue layer is reduced from 200-700 V/cm to 20-50 V/cm under the condition of maintaining equally transduction efficiency with the normal electrode, and greatly reducing the injury to the muscular tissues. The invention has the advantages of small size, light weight, adjustable parameters, friendly clinical application interface, etc., thereby realizing safe and efficient vivo gene transduction, and providing a new method and a new tool for further carrying on clinical gene therapy tests and researches.

Description

technical field [0001] The invention relates to an experimental instrument for clinical medical research, especially an in vivo gene transduction device, which can provide efficient and safe gene transduction, and provide a new method and tool for further gene therapy. Background technique [0002] The effect of gene therapy is closely dependent on whether the transduced gene can be safely and effectively delivered to the target organ tissue. Among gene vectors, there are viral vectors and non-viral vectors. Among them: the viral vector plays an important role in the process of carrying the virus into the cell and causing the toxicity of the cell infection. However, it has recently been reported that hemophilia with AAV vectors can block viral vector entry into cells through a counteractive immune response (1, 2). In addition, the combination of the viral vector and the host genome may cause the virus to insert into the chromosome, causing serious consequences. According ...

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Problems solved by technology

However, this approach can lead to irreversible damage to the tissue (19, 20)

According to the research report, the damage degree of this method is much higher than that caused by the current of 100V / cm, and it can even directly lead to cell death (21)

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