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Carbon dioxide diagnosis/ determination reagent kit and carbon dioxide concentration determination method

A technology of carbon dioxide and kit, which is applied in the field of carbon dioxide concentration measurement and carbon dioxide diagnosis/measurement kit, which can solve the problems of lack of experimental research and so on

Inactive Publication Date: 2008-04-30
SUZHOU ANJ BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0013] The above patents have nothing to do with enzymatic determination, which shows from one aspect that domestic experimental research in this area is very lacking

Method used

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  • Carbon dioxide diagnosis/ determination reagent kit and carbon dioxide concentration determination method

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Embodiment 1

[0036] The carbon dioxide diagnosis / measurement reagent of this embodiment is a single reagent, including:

[0037] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0038] Stabilizer 50mmol / L

[0039] Reduced coenzyme 0.25mmol / L

[0040] Malate dehydrogenase 8000U / L

[0041] Pyruvate 12mmol / L

[0042] After the reagents are all dissolved and prepared, they are divided into bottles and freeze-dried to make dry powder reagents; before use, add purified water and reconstitute for use.

[0043] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide sample to the reagent is 1 / 25, The reaction direction is negative reaction (decreasing reaction), the detection method is the endpoint method, the delay time is about 0 minutes, and the detection time is about 5 minutes.

[0044] After adding the...

Embodiment 2

[0046] The carbon dioxide diagnosis / measurement reagent of this embodiment is a double reagent, including:

[0047] Reagent 1

[0048] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0049] Stabilizer 50mmol / L

[0050] Reduced coenzyme 0.25mmol / L

[0051] Pyruvate 12mmol / L

[0052] Reagent 2

[0053] Tris(carboxymethyl)aminomethane-hydrochloric acid buffer 100mmol / L

[0054] Stabilizer 50mmol / L

[0055]Malate dehydrogenase 8000U / L

[0056] After the reagents are all dissolved and prepared, they are divided into bottles to make liquid double reagents, which can be used directly.

[0057] Set on the automatic biochemical analyzer: temperature 37°C, reaction time 10 minutes, initial absorbance 1.8±0.7, test main wavelength 340nm, test secondary wavelength 405nm, the volume ratio of the measured carbon dioxide sample to reagent 1 and reagent 2 is 2 / 20 / 5, the reaction direction is negative reaction (decreasing reaction), the detection method is the rate...

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Abstract

The invention relates to a carbon dioxide diagnosing / testing reagent box which utilizes the enzyme color method, and also relates to a method for testing the consistence of carbon dioxide, and the elements and the components of reagent. The invention belongs to the technical field of the medicine / food / environment check. The reagent box of the invention mainly comprises cushion fluid, pyruvic acid, malate dehydrogenase (codecarboxylase), reduced enzyme and stabilizer. A series of enzyme reactions occur by mixing the sample and the reagent according to a certain volume rate. Then the reactant is arranged under an ultraviolet and visible light analysis instrument to test the reduction extend and the speed of the absorbency at the 340nm of the main wavelength, thereby testing the consistence of the carbon dioxide. By adopting the invention, the testing result can be obtained by the ultraviolet and visible light analysis instrument, and the promotion and the application are convenient.

Description

technical field [0001] The invention relates to a carbon dioxide diagnosis / measurement kit, and at the same time, the invention also relates to a method for measuring carbon dioxide concentration, which belongs to the technical field of medicine / food / environmental inspection and measurement. Background technique [0002] The content of carbon dioxide in the blood plays an important role in the regulation of acid-base balance in the human body. The constant blood pH is an essential condition for maintaining life activities. Among the various buffer systems in blood plasma, the bicarbonate buffer system is the most important, which directly affects the pH of blood. [0003] Changes in the content of carbon dioxide in the blood mainly reflect metabolic acid-base balance disorders. In pure metabolic acid or alkalosis, blood bicarbonate [HCO 3 - ] decreased or increased, the total carbon dioxide also decreased or increased. In simple respiratory acid or alkalosis, blood bicar...

Claims

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Application Information

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IPC IPC(8): G01N33/84G01N21/78G01N21/25C12Q1/32
Inventor 王尔中
Owner SUZHOU ANJ BIOTECHNOLOGY CO LTD
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