Edible mythic fungus bacterium ball milk and preparation method thereof
A technology of ganoderma bacteria and ganoderma, applied in food preparation, milk preparations, applications, etc., can solve the problems of high cost, incomplete content of effective components in ganoderma milk, complicated processing, etc., and achieve low cost, easy promotion and implementation, and good taste Good results
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Embodiment 1
[0048] 1. Take out the refrigerated Ganoderma lucidum (Ganoderma lucidum (Leyss.ex Fr.) Karst.CCTCCNo.AF92001), put it at room temperature for 2 days, and transfer it to the PDA slant medium.
[0049] 2. Keep the temperature at 28°C, culture for 4-6 days, and the white ganoderma lucidum mycelium will cover the inclined surface before use.
[0050] 3. Make CD-06 culture solution, put it into a 1000ml triangular flask, 200ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2 , keep the pressure for 40 minutes. After sterilization, it can be used for inoculation after natural cooling to 30°C.
[0051] 4. Insert 2-4 cm into each of the above-mentioned triangular flasks 2 The fresh and pure Ganoderma lucidum slant is cultivated at a culture temperature of 28°C to 30°C and a rotating shaker at a speed of 200 rpm for 4 to 6 days. After the culture liquid is clear and the bacteria balls are formed, the seed liquid is made.
[0052] 5. Make CS-15 cul...
Embodiment 2
[0058] 1. Make CD-06 culture solution, put it into a 1000ml triangular flask, 300ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2 , keep the pressure for 40 minutes. After sterilization, it can be used for inoculation after natural cooling to 30°C.
[0059] 2. Insert 2-4 cm into each of the above-mentioned triangular flasks 2 The fresh and pure ganoderma lucidum (W.Curtis: Fries) Karsten CGMCC No.5.706 bacterial slant was cultivated at a temperature of 30°C and a rotating shaker at a speed of 180 rpm for 4 to 6 days. Once formed, the seed solution is made.
[0060] 3. Make CS-15 culture solution, put it into a 5000ml Erlenmeyer flask, 2000ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2 , keep the pressure for 50 minutes. After sterilization, it can be used for inoculation after natural cooling to 30°C.
[0061] 4. Put a bottle of liquid Ganoderma lucidum in a 1000ml Erlenmeyer flask into a steril...
Embodiment 3
[0063] 1. Strain selection: Ganoderma lucidum (W.Curtis: Fries) Karsten.CGMCC No.5.744;
[0064] 2. Slant culture: inoculate the bacteria on the PDA slant medium, culture at 28°C-30°C for 4-6 days;
[0065] 3. Make CD-06 culture solution, put it into a 1000ml triangular flask, 320ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2 , keep the pressure for 40 minutes. After sterilization, it can be used for inoculation after natural cooling to 30°C.
[0066] 4. Insert 2-4 cm into each of the above-mentioned triangular flasks 2 The fresh and pure Ganoderma lucidum slant was cultivated at a culture temperature of 29°C and a rotating shaker at a speed of 170 rpm for 4 to 6 days. After the culture medium was clear and the bacteria balls were formed, the seed solution was made.
[0067] 5. Make CS-15 culture solution, put it into a 5000ml Erlenmeyer flask, 2000ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2...
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