Edible pupa aweto bacterium ball milk and preparation method thereof
A technology of Cordyceps militaris and a production method, which are applied in the directions of food preparation, milk preparations, applications, etc., can solve the problems of high price, shortage of Cordyceps militaris resources, inconvenience in eating, etc., and achieve the effects of low cost, easy promotion and implementation, and intuitive form.
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Embodiment 1
[0043] 1. Take out the refrigerated Cordyceps militaris CCTCC No.M88061, put it at room temperature for 2 days, and then transfer it to the PDA slant medium.
[0044] 2. Keep the temperature at 25°C, cultivate for 4 to 6 days, and use it after the hyphae cover the inclined surface.
[0045] 3. Make CC-06 culture solution, put it into a 1000ml triangular flask, 200ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2 , keep the pressure for 40 minutes. After sterilization, it can be used for inoculation after natural cooling to 25°C.
[0046] 4. Insert 2-4 cm into each of the above-mentioned triangular flasks2 The fresh and pure Cordyceps militaris slant was cultivated at a culture temperature of 20°C and a rotating shaker at a speed of 200 rpm for 4 to 6 days. After the culture liquid was clear and the bacteria balls were formed, the seed liquid was made.
[0047] 5. Make CC-15 culture solution, put it into a 5000ml Erlenmeyer flask, 2000ml / b...
Embodiment 2
[0053] 1. Make CC-06 culture solution, put it into a 1000ml triangular flask, 300ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2 , keep the pressure for 40 minutes. After sterilization, it can be used for inoculation after natural cooling to 25°C.
[0054] 2. Insert 2-4 cm into each of the above-mentioned triangular flasks 2 The fresh and pure Cordyceps militaris (Cordyceps militaris) CCTCC No.M88061 bacterial slant was cultured at a temperature of 25°C and a rotating shaker at a speed of 180 rpm for 4 to 6 days. After the culture liquid became clear and the bacteria balls formed, the seeds were made liquid.
[0055] 3. Make CC-15 culture solution, put it into a 5000ml Erlenmeyer flask, 2000ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2 , keep the pressure for 50 minutes. After sterilization, it can be used for inoculation after natural cooling to 25°C.
[0056] 4. Put one bottle of the liquid ...
Embodiment 3
[0058] 1. Strain selection: Cordyceps militaris CCTCC No.M88061;
[0059] 2. Slant culture: inoculate the bacteria on the PDA slant medium, culture at 22°C-24°C for 4-6 days;
[0060] 3. Make CC-06 culture solution, put it into a 1000ml triangular flask, 320ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, pressure 1kg / cm 2 , keep the pressure for 40 minutes. After sterilization, it can be used for inoculation after natural cooling to 25°C.
[0061] 4. Insert 2-4 cm into each of the above-mentioned triangular flasks 2 The fresh and pure Cordyceps militaris (Cordyceps militaris) CCTCC No.M88061 bacterial slant was cultured at 24°C and the rotation speed of the rotary shaker was 170 rpm for 4 to 6 days, and the seeds were made after the culture liquid was clear and the bacteria balls were formed. liquid.
[0062] 5. Make CC-15 culture solution, put it into a 5000ml Erlenmeyer flask, 2000ml / bottle, cover with 8 layers of gauze, sterilize with steam, 121℃, p...
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