Preparation method for tobacco extract
A technology of tobacco extract and tobacco, which is applied in the direction of tobacco, tobacco treatment, application, etc., can solve the problems of physical indicators not meeting the production and processing requirements, complex preparation process and equipment used, hindering the development of the tobacco industry, etc., and achieve excellent stability and Reproducibility, simplicity of method, enhanced performance
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Examples
Embodiment 1
[0026] Reference figure 1 , (1) First, add water and enzyme to the tobacco powder for enzymatic hydrolysis: the pulverization degree of tobacco leaf is 40 mesh, the ratio of material to water is 1:4, the enzyme used is cellulase, the dosage is 2‰ of the mass of tobacco leaf, the enzymatic hydrolysis time is 14hr, The hydrolysis temperature is 40℃; then trypsin and acid protease are added, the dosage is 2‰ of the tobacco leaf mass, the enzymolysis time is 14hr, and the enzymolysis temperature is 40℃.
[0027] (2) Ethanol extraction, filtration and concentration: Add absolute ethanol to (1) to an ethanol concentration of 30%, reflux extraction with condenser, extraction time 4hr, after extraction, filter, use a plate and frame filter to filter the filtrate Suction filtration is performed again to remove insoluble impurities in the extract; the extract should be concentrated by a thin-film evaporator that can be fed continuously. The evaporation conditions are the condensation tempe...
Embodiment 2
[0030] Reference figure 1 , (1) First, add water and enzyme to the tobacco powder for enzymolysis: the pulverization degree of tobacco leaves is 200 mesh, the ratio of material to water is 1:15, the enzyme used is arabinase, the dosage is 8‰ of the quality of tobacco leaves, and the enzymolysis time is 6hr. The enzymolysis temperature is 70°C; then the compound protease and trypsin are added, the dosage is 8‰ of the tobacco leaf quality, the enzymolysis time is 6hr, and the enzymolysis temperature is 70°C.
[0031] (2) Ethanol extraction, filtration and concentration: Add absolute ethanol to (1) Enzymatic hydrolysis solution to an ethanol concentration of 75%, reflux extraction with a condenser, extraction time 1hr, after extraction, filter, and filter the filtrate once more. The insoluble impurities in the extract are removed; the extract is concentrated by a thin-film evaporator that can be fed continuously. The evaporation conditions are 2°C for condensation and 80°C for heati...
Embodiment 3
[0034] Reference figure 1 , (1) First, add water and enzyme to the tobacco powder for enzymolysis: the pulverization degree of tobacco leaves is 80 mesh, the ratio of material to water is 1:7, the enzyme used is hemicellulase, the dosage is 4‰ of the quality of tobacco leaves, and the enzymolysis time is 12hr. The enzymolysis temperature is 60℃; then alkaline protease and neutral protease are added, the dosages are respectively 4‰ and 5‰ of the tobacco leaf quality, the enzymolysis time is 10hr, and the enzymolysis temperature is 55℃.
[0035] (2) Ethanol extraction, filtration and concentration: Add absolute ethanol to (1) until the ethanol concentration is 50%, and the condenser refluxes for extraction. The extraction time is 2 hours. After extraction, filter and filter the filtrate again. Insoluble impurities are removed; the extract is concentrated by a thin-film evaporator that can be fed continuously. The evaporation conditions are 5°C for condensation and 70°C for heating....
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Description
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Application Information
- IPC
- A24B15/18; A24B15/20; A24B15/24
- Inventors
- 饶国华; 温东奇