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Medlar HPLC fingerprint establishment method and its standard fingerprint

A standard fingerprint and fingerprint technology, applied in the field of drug analysis, can solve the problems of lack of wolfberry quality and achieve good stability and reproducibility

Inactive Publication Date: 2012-01-04
BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, people's quality control of wolfberry is limited to qualitative and quantitative analysis of an active component or an index component among its many components, and there is a lack of standard control methods that fully reflect the quality of wolfberry.

Method used

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  • Medlar HPLC fingerprint establishment method and its standard fingerprint
  • Medlar HPLC fingerprint establishment method and its standard fingerprint

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Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0016] Preferred embodiments of the present invention are as follows:

[0017] (1) Instruments and medicines: Shimadzu LC10A high performance liquid chromatograph was used; 21 batches of wolfberry medicinal materials were purchased from Chinese herbal medicine stores; other reagents were of chromatographic or analytical grade.

[0018] (2) Preparation of the test solution: Precisely weigh 10 g of medicinal powder, put it in a volumetric flask, add 500 mL of 80% methanol, ultrasonically shake for 60 min, and cool to constant volume. The supernatant was filtered with a 0.45 μm microporous membrane, and the filtrate was used as the test solution.

[0019] (3) High performance liquid chromatography analysis: The packing of the chromatographic separation column is 5μm Kromasil-C18, 250×4.6mm; the mobile phase is water-acetonitrile; the gradient elution method is adopted: 0min→15min→30min→45min→60min, acetonitrile 10% →22%→35%→45%→55%; flow rate 1.0mL / min; detection wavelength 254n...

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Abstract

The invention provides a method for establishing an HPLC fingerprint spectrum of Fructus Lycii and a standard fingerprint spectrum thereof. The method comprises the following steps of: preparing a test solution, preparing a reference solution, selecting chromatographic conditions, and carrying out HPLC determination to obtain the fingerprint spectrum of Fructus Lycii. The standard fingerprint spectrum of Fructus Lycii can be obtained by comparing HPLC fingerprint spectra of 21 batches of Fructus Lycii materials and determining the common fingerprint characteristics. The method has the advantages of simple operation, good reproducibility, a plurality of characteristic peaks, accurate and reliable result, etc. The standard fingerprint spectrum can be used for effectively monitoring the quality of Fructus Lycii.

Description

technical field [0001] The invention relates to a method for establishing fingerprints of Chinese medicinal materials, in particular to a method for establishing high performance liquid chromatography (HPLC) fingerprints of Chinese medicinal materials, and the HPLC standard fingerprints of Lycium barbarum obtained by the method. It belongs to the technical field of drug analysis. Background technique [0002] The fingerprint of traditional Chinese medicine refers to the spectrum or image of the group characteristics of each component of traditional Chinese medicine obtained by modern analysis techniques such as spectrum or chromatography. At present, fingerprints have become one of the most effective means to identify Chinese medicine varieties and evaluate the quality of Chinese medicines recognized at home and abroad. In the "FDA Guidelines on Botanical Products" formulated by the US Food and Drug Administration in 1996, it is required to provide corresponding fingerprint...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/86G01N30/06G01N30/02G01N33/15
Inventor 周考文王树聪李凤桐
Owner BIOCHEM ENG COLLEGE OF BEIJING UNION UNIV