Reagent kit for detecting individual oxidation resistance genetic susceptibility

A kit, anti-oxidation technology, applied in the direction of measurement devices, biochemical equipment and methods, microbe determination/inspection, etc., can solve the problems of increased release rate into blood vessels, decreased binding capacity of heparin, etc.

Inactive Publication Date: 2008-08-13
SHANGHAI HENGJIAN BIOTECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Variation C does not affect the enzyme activity of SOD3, but it reduces the binding ability of heparin,

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Example 1. Use of detection kits

[0031] Step 1: Extraction of DNA template

[0032] Genomic DNA of oral epithelial cells was extracted by silica gel adsorption.

[0033] Step 2: Real-time quantitative PCR reaction

[0034] Use a fluorescent quantitative PCR detection kit for individual genetic susceptibility to antioxidant, which contains the following primer pairs and fluorescent probe pairs:

[0035] Sense primer 1: 5'-TTTCAAAATTCCTGCTTAC-3' (SEQ ID NO: 1)

[0036] Antisense primer 1: 5'-AGAAATCTGCTTCCCC-3' (SEQ ID NO: 2)

[0037] Sense primer 2: 5'-TCCTCCCTCCC-3' (SEQ ID NO: 3)

[0038] Antisense primer 2: 5'-AACAGCTTCACCAC-3' (SEQ ID NO: 4)

[0039] Sense primer 3: 5'-CCCTGCTGCTGC-3' (SEQ ID NO: 5)

[0040] Antisense primer 3: 5'-GGGGCAGAAGGAA-3' (SEQ ID NO: 6)

[0041] Sense primer 4: 5'-GGAGCACTCAGAGC-3' (SEQ ID NO: 7)

[0042] Antisense primer 4: 5'-GCCTTGCACTCGCTC-3' (SEQ ID NO: 8)

[0043] Sense primer 5: 5'-GGACCTGAGCACTTTTATG-3' (SEQ ID NO: 9)

[...

Embodiment 2

[0064] Example 2. Services for testing individuals' genetic susceptibility to antioxidants

[0065] Step 1: DNA Extraction

[0066] The physicians in the laboratory department of the hospital instructed the subjects to use oral swabs to sample oral epithelial cells, and the silica gel adsorption method was used to extract DNA from oral epithelial cells.

[0067] Step 2: Genotyping Assays

[0068]Using the kit provided by the present invention, the SNP site No. rs769214 on the catalase gene, the SNP site No. rs4673 on the reduced coenzyme phytol gene, and the endothelial nitric oxide synthase gene on the genomic DNA of the subject were tested. The SNP site rs1799983, the SNP site rs1799895 on the extracellular superoxide dismutase gene, and the SNP site rs662 on the paraoxonase 1 gene were detected by fluorescent quantitative PCR to determine the identity of the five SNPs sites. genotype.

[0069] Step 3: Analysis of Individual Antioxidant Genetic Susceptibility

[0070] Th...

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PUM

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Abstract

The invention discloses a kit for detecting individual oxidation resistance genetic predisposition, comprising: a specific primer pair for simultaneous detection of No.rs769214 SNP locus on catalase gene (CAT), No.rs4673 SNP locus on NADPH phytol gene (CYBA), No.rs1799983 SNP locus on endodermis NOS gene (NOS3), No.rs1799895 SNP locus on ectoenzyme peroxide dismutase gene (SOD3), and No.rs662 locus on paraoxonase-1 gene (PON1); a specific fluorescent probe pair; an ordinary component for fluorescent quantitative PCR detection, etc. The kit of the invention estimates individual oxidation resistance genetic predisposition by simultaneous detecting mononucleotide polymorphism locus genes of CAT, CYBA, NOS3, SOD3, PON1 closely related to the oxidation resistance ability.

Description

technical field [0001] The present invention relates to the fields of molecular biology and medicine, and more specifically, the present invention relates to a kit for detecting the genetic susceptibility of an individual to antioxidant. Single nucleotides of prototypic coenzyme phytol gene (CYBA), endothelial nitric oxide synthase gene (NOS3), extracellular superoxide dismutase gene (SOD3), paraoxonase 1 gene (PON1) Polymorphic locus genotypes to assess individual genetic susceptibility to antioxidants. Background technique [0002] There is a very important balance system in the human body: oxidation-reduction system. This system regulates the production and metabolism of oxygen free radicals in the body, maintains the proper oxidation capacity and scavenging ability of oxygen free radicals in the body, and thus ensures the progress of life activities. The imbalance of oxidation-reduction system and the accumulation of excessive oxygen free radicals will cause a series o...

Claims

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Application Information

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IPC IPC(8): G01N21/00C12Q1/68
Inventor 冯哲民邹祖烨
Owner SHANGHAI HENGJIAN BIOTECH CO LTD
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