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Construction and use of Q-type human paraoxonase 1 gene expression vector

A paraoxonase and gene technology, applied in the field of biotechnology and pharmaceuticals, can solve the problems of multiple medications, short action time, slow onset, etc., and achieve significant liver protection effect, fast onset, and less toxic and side effects

Inactive Publication Date: 2007-05-16
NANJING UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, most of them have the disadvantages of short action time, slow onset, and need for long-term multiple medications.

Method used

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  • Construction and use of Q-type human paraoxonase 1 gene expression vector

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Embodiment Construction

[0028] 1. The pBluescript-hPON1Q plasmid was donated by Professor C.E. Furlong. Using pBluescript-hPON1Q as a template, use primers 1 and 2 to amplify the full-length gene of hPON1Q with a total of 1065 bp by PCR method, and insert it into the multiple cloning site of pcDNA3.0 plasmid after double digestion with EcoR I and HindIII. The hPON1Q gene is under the control of the CMV promoter. Sequencing proved that the result was completely consistent with the designed sequence (see Figure 1 for the process flow). Transform pcDNA3.0-hPON1Q with CaCl 2The competent TOP10 Escherichia coli was prepared by the method, and the Escherichia coli was amplified according to the operation procedure of the third edition of "Molecular Cloning" to extract a large number of plasmids. The purity and yield of the extracted plasmids were determined by UV spectrophotometry, and the plasmid concentration was adjusted to 1 μg / μl with PBS. All biochemical reagents were purchased from Sigma, and enz...

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Abstract

The invention discloses a recombined Q-typed hPON1Q in the biological pharmaceutical technological domain, which is characterized by the following: transmitting expressive plasmid to do ectopic secretory expression through skeletal muscle excited by electricity; improving serum PON1 level effectively; preventing liver from damaging as new gene drug; maintaining the activity of phosphoroso enzyme over 16d; making the genome-switching mouse serum ALT and AST level lower than positive contrast group (P<0.01).

Description

1. Technical field [0001] The invention belongs to the technical field of biotechnology and pharmacy. 2. Background technology [0002] Paraoxonase 1 (PON1) belongs to the paraoxonase gene family and is a broad-spectrum hydrolase dependent on calcium ions. Its chemical essence is a glycoprotein with a molecular weight of 43-54 kDa composed of 354 amino acids [Zhu J, et al. Appl Microbiol Biotechnol, 2006, 72: 103-108]. Paraoxonase 1 in mammals is synthesized by liver cells and is mainly distributed in blood, liver, kidney and other tissues and organs. PON1 exhibits aryl esterase activity, lactonase activity, and organophosphatase activity on a variety of substrates in vitro. PON1 in serum is mainly combined with high-density lipoprotein, and the enzyme activity is increased by more than 40 times after binding. The main physiological function of PON1 is to hydrolyze oxidized lipids in low-density lipoprotein, thereby reducing the oxidative stress of the body. PON1 activit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/85C12N15/52A61K31/7088A61P1/16
Inventor 秦浚川张驰朱洁臧宇辉彭薇姜晓玲
Owner NANJING UNIV
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