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Process for producing bos taurus domesticus gmelin enzyme crude product

A crude, ox-mouth technology, applied in biochemical equipment and methods, enzymes, enzymes, etc., can solve the problem of no ox-mouth enzyme products, etc., and achieve the effect of stable process and high yield

Inactive Publication Date: 2008-08-20
焦丽 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The preparation method of the crude bovine sialase product of the present invention solves the problem that there is no bovine oral sialase product at present, and its product is convenient for biochemical research and application of biological products

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0026] The bovine sialolytic enzyme crude product raw material of the present invention is as follows:

[0027] Beef saliva 1 kg

[0028] 5mol / L HCl about 20ml

[0029] 0.6mol / L ZnCl 2 330ml (about 0.027kg ZnCl 2 )

[0030] 5mol / L NaOH about 50ml

[0031] Production process:

[0032] The preparation conditions are that the temperature of cattle saliva is less than 10°C, filter the collected cattle saliva with a 40-mesh sieve to remove impurities, take the filtrate, add 5mol / L HCl under stirring, adjust the pH value to 4-5, add 0.6mol / L ZnCl 2 Appropriate amount, then use 5mol / L NaOH to adjust the pH value to 8-9 under stirring, centrifuge or filter, discard the supernatant or filtrate, take the precipitate, and adopt natural air-drying or low-temperature drying method (air drying, freeze-drying) at less than 40°C. Moisture is less than 16%, promptly obtains about 10 grams of the crude product of bovine oral sialase.

[0033] Moisture analysis method: the dry...

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Abstract

The invention pertains to the technical field of biochemical reagent enzymes, in particular to a preparation method of a cattle sialidase crude product, and the product can facilitate the biochemical research and the application of the biological products. The preparation method prepares the crude cattle sialidase by neutral salt zinc chloride, which has the advantages of stable technique, high yield and convenient operation, and the preparation method is applicable to wide grassroots farmers for extracting the crude enzyme from cattle saliva.

Description

Technical field: [0001] The invention belongs to the technical field of biochemical reagents and enzymes, and solves the problem that there are no bovine mouth saliva enzyme products at present. In particular, it relates to a method for preparing a crude product of bovine oral sialase. Background technique: [0002] At present, there are very few studies on the composition and mechanism of action of cattle saliva in my country, and there are no invention patents on cattle saliva enzyme products. According to data, saliva is secreted by many large and small salivary glands all over the depth of oral mucosa. There are three pairs of large saliva: parotid gland, submandibular gland, and sublingual gland, and small glands such as lip gland, tongue gland, buccal gland, and palatine gland. More than 95% of saliva is water, and there are amylase, lysozyme, peroxidase, catalase, mucin, globulin, adrenal cortex hormone, hyperglycemia, vitamin C, amino acids, calcium phosphate, Pot...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/00C12N9/98
Inventor 焦丽王有为
Owner 焦丽