Detection kit for bacterial vaginosis (BV) and sialidase detection method

Abstract

The purpose of the invention is to provide a detection kit for BV, and the detection kit has the advantages of a simple testing process, strong pertinency, high accuracy and fast and convenient operation. The technical scheme for the invention is that a detection kit for BV is provided and comprises a reaction solution and a colored solution, wherein the reaction solution comprises specific zymolytes, carbohydrates and a potassium acetate buffer, and the zymolytes are n-acetylneuraminic acid and salts thereof. The beneficial effect of the invention is simplification of testing methods and result interpretation of BV diagnosis. Discrimination with the sialidase detection method has the following advantages: 1, testing time is substantially saved, all the testing operation is finished with ten minutes or more, no other complex equipment is needed, and only a baking box or a culture case at a temperature of 37 DEG C is needed to provide; 2, the interpretation step is rapid and visual, and the rate of misoperation and misdiagnosis are greatly reduced; 3, the detection card is sensitive, accurate and convenient, and has a high accordance rate with the detection of BV by golden standard the Amsel method.

Description

technical field [0001] The invention relates to the detection of bacterial vaginosis, in particular to a detection kit and a sialidase detection method of bacterial vaginosis. Background technique [0002] Bacterial vaginosis (BV) is one of the most common lower genital tract infections among married women. It is a clinical syndrome caused by an increase in the number of a group of anaerobic bacteria (Bacteroides, Gardnerella, Mobilonia, Mycoplasma hominis, and Peptostreptococcus, etc.). BV is one of the common gynecological diseases, the infection rate is 15-50%, and it is easy to recur. 50% of women suffering from BV are prone to premature delivery or low birth weight babies, and their babies are also likely to have various sequelae. More and more evidence shows that BV is a risk factor for organizing choriovitis, amniotic fluid infection, post-cesarean endometritis, and other adverse pregnancy and pregnancy complications. In addition, in gynecology, BV is mainly relate...

AI Technical Summary

Problems solved by technology

Therefore, Amsel diagnostic criteria are not easy to grasp and are easily affected by human factors. However, the use of bacterial culture is cumbersome and time-consuming, which is not conducive to timely diagnosis and treatment of patients, and limits the popularization and application.

[0005] In the diagnosis of BV, the most commonly used clinical method is the traditional Amsel method (as mentioned above), but it is not easy to implement it accurately in practice

First of all, some factors not related to infection, such as recent sexual intercourse, vaginal douching, menstruation, or the presence of a large amount of cervical mucus, can also lead to an increase in vaginal pH or odor of secretions; the identification of odor in the amine test depends on the individual. However, the identification of clue cells, which are the best markers for BV diagnosis, is affected by many factors such as the quality of microscopic equipment, sample collection, and operator experience, and is highly subjective, so it is difficult to judge the results. Statistics and Comparisons