Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Alzheimer''s disease early diagnosis liquid phase chip and method for producing the same

A technology for Alzheimer's disease and early diagnosis, which is applied in the field of a liquid-phase chip for early diagnosis of Alzheimer's disease and its preparation, and achieves the effects of a simple and easy preparation method, high sensitivity and high detection efficiency

Inactive Publication Date: 2008-08-20
SUREXAM BIO TECH
View PDF0 Cites 8 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] The purpose of the present invention is to provide an Alzheimer’s disease that can detect five kinds of markers in order to address the lack of convenient and effective early diagnosis methods for Alzheimer’s disease and the defect that the existing technology can only detect one neurobiochemical marker at a time. Alzheimer's disease early diagnosis liquid chip, the liquid chip provides an efficient, accurate and simple clinical detection method for the early diagnosis of Alzheimer's disease

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Alzheimer''s disease early diagnosis liquid phase chip and method for producing the same
  • Alzheimer''s disease early diagnosis liquid phase chip and method for producing the same
  • Alzheimer''s disease early diagnosis liquid phase chip and method for producing the same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0064] Example 1 Preparation of liquid chip for early diagnosis of Alzheimer's disease and detection of antigen

[0065] The capture antibody of the present invention can correspond to t-tau, p-tau231, p-tau181, p-tau199 and Aβ respectively. 1-42 Specific binding monoclonal antibodies; the detection antibodies are respectively capable of interacting with t-tau, p-tau231, p-tau181, p-tau199 and Aβ 1-42 Specific binding monoclonal antibody or polyclonal antibody.

[0066] T-tau, p-tau231, p-tau181, p-tau199 and Aβ used in this embodiment 1-42 The capture antibody and detection antibody were purchased from Jingmei Bioengineering Co., Ltd.

[0067] In this embodiment, the formulations of the various solutions are as follows:

[0068] 1.50mM MES buffer (pH5.0) formula (250ml):

[0069] Reagent

Source

Final concentration

Dosage per 250ml

MES

(2[N-Morpholino]

ethanesulfonic

acid)

Sigma M-2933

0.05M

2.44g

...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an alzheimer early diagnosis liquid chip which mainly includes the following components: coating microsphere, microsphere coated with t-tau capture antibody, microsphere coated with p-tau231 capture antibody, microsphere coated with p-tau181 capture antibody, microsphere coated with p-tau199 capture antibody, microsphere coated with A beta 1-42 capture antibody, these microspheres have codes with different colors; biotin-labeled test antibody; avidin-linked phycoerythrin. The invention alzheimer early diagnosis liquid chip has advantages of high detection efficiency, a small amount of sample, high specificity and high sensitivity. And at the same time, every neural biochemical injury marker can freely assemble for convenient usage. At the same time, because all the reactions are in liquid environment, which is good for maintaining native conformation of protein, the reaction between probe and tested material is faster and more complete, so the test sensitivity and the linear range are improved significantly.

Description

Technical field [0001] The present invention relates to medical in vitro diagnostic technology, in particular to a liquid phase chip for early diagnosis of Alzheimer's disease and a preparation method thereof. Background technique [0002] Senile dementia (also known as Alzheimer's disease, AD) is a neurodegenerative disease with clinical manifestations of cognitive decline, life function decline, and abnormal mental behavior. The typical pathological features of AD are neurofibrillary tangles (NFT) in neurons, senile plaques (SP) deposits outside the cells and the number of neurons decreases. With the increase of the aging population, the prevalence of AD is increasing year by year. Currently, about 18 million people worldwide suffer from Alzheimer's disease, 50% of which occur in developing countries. According to WHO estimates, by 2050, the number of people suffering from the disease in the world will double to 34 million. Statistics show that the prevalence of Alzheimer's dis...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N33/543G01N33/546
Inventor 许嘉森林一群
Owner SUREXAM BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com