Combined splint microelectrode type micro-fluidic dielectrophoresis cell separation and enrichment chip

Abstract

The invention discloses a combined splint microelectrode type micro-fluidic dielectrophoresis cell separation and enrichment chip and relates to a micro-fluidic chip which is applied in cell sample separation and enrichment, a dielectrophoresis (DEP ) splint combined type electrode is obtained through depositing gold film on the surface of glass, and the electrode can be connected with a corresponding external connected low pressure high frequency ac circuit to realize DEP separation and enrichment process control. The combined splint microelectrode which is provided by the invention designs that the functions of on-line separation and on-line enrichment of sample such as cells and the like are effectively integrated on a chip, which realizes organic combination between the micro-fluidic chip technique and the microelectrode principles, and the chip can be applied and can realize the separation and the enrichment of various cells and bacterium.

Description

Technical field: [0001] The invention belongs to the integrated micro-biochemical analysis system technology, in particular to a microfluidic chip for cell separation and enrichment. Background technique: [0002] In the fields of chemistry and biology, the separation of cell-based biological sample systems is a technique to obtain uniform target cells from a large number of heterogeneous cell sample populations, and is often used in cell biology, clinical medicine, and drug development, etc. It is especially important for the detection, diagnosis and treatment of diseases. Among the cell separation technologies and methods that use microfluidic chips as a technical platform, integrated microfluidic dielectrophoresis (DEP) chips have become a new biochemical analysis technology that researchers at home and abroad are very concerned about. At present, relevant researches only use dielectrophoresis (DEP) as the means to achieve the purpose of cell enrichment, and the chip res...

AI Technical Summary

Problems solved by technology

Traditional DEP chip research mainly focuses on the static separation and enrichment of particles and microscopic observation through the design of array microelectrodes. For the integration of microfluidic technology and particle enrichment and separation process, the qualitative and quantitative analysis of the characteristic parameters of particles in the DEP process The application of DEP chips to bacteria and cell systems to achieve quantitative detection is still in its infancy, and people are paying great attention to the research on the broader goals of biochemical detection

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