Combined splint microelectrode type micro-fluidic dielectrophoresis cell separation and enrichment chip

A technology of dielectrophoresis, separation and enrichment, which is applied in the direction of bioreactor/fermenter combination, enzymology/microbiology device, stress-stimulated microbial growth method, etc. It can solve problems that need to be further studied and achieve separation and enrichment Low voltage, no damage to the biological activity of the sample, and low power consumption

Inactive Publication Date: 2008-08-27
CHONGQING UNIV
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  • Abstract
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Problems solved by technology

Traditional DEP chip research mainly focuses on the static separation and enrichment of particles and microscopic observation through the design of array microelectrodes. For the integration of microfluidic technology and particle enrichment and separation process, the qualitative and quantitat

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  • Combined splint microelectrode type micro-fluidic dielectrophoresis cell separation and enrichment chip
  • Combined splint microelectrode type micro-fluidic dielectrophoresis cell separation and enrichment chip
  • Combined splint microelectrode type micro-fluidic dielectrophoresis cell separation and enrichment chip

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Embodiment Construction

[0015] The technical solution of the present invention will be further described in detail through specific embodiments according to the accompanying drawings in the description.

[0016] figure 1 It is a schematic diagram of the splint-type microfluidic dielectrophoresis cell separation and enrichment chip of the present invention. The combined splint microelectrode type microfluidic dielectrophoresis cell separation and enrichment chip structure includes: first and second sets of splint electrodes 4-1 and 4-2, sample injection separation pipeline 3 and enrichment pipeline 6. see figure 2 , the sample injection separation pipeline 3 is a Y-shaped pipeline, the port is connected with the liquid inlet pool 2 and two buffer solution pools 1, there are three branch pipelines at the end of the separation pipeline, the middle one is the sample enrichment pipeline 6, and the port is connected with the sample pool 7 , and the remaining two pipelines are directly connected to the w...

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Abstract

The invention discloses a combined splint microelectrode type micro-fluidic dielectrophoresis cell separation and enrichment chip and relates to a micro-fluidic chip which is applied in cell sample separation and enrichment, a dielectrophoresis (DEP ) splint combined type electrode is obtained through depositing gold film on the surface of glass, and the electrode can be connected with a corresponding external connected low pressure high frequency ac circuit to realize DEP separation and enrichment process control. The combined splint microelectrode which is provided by the invention designs that the functions of on-line separation and on-line enrichment of sample such as cells and the like are effectively integrated on a chip, which realizes organic combination between the micro-fluidic chip technique and the microelectrode principles, and the chip can be applied and can realize the separation and the enrichment of various cells and bacterium.

Description

Technical field: [0001] The invention belongs to the integrated micro-biochemical analysis system technology, in particular to a microfluidic chip for cell separation and enrichment. Background technique: [0002] In the fields of chemistry and biology, the separation of cell-based biological sample systems is a technique to obtain uniform target cells from a large number of heterogeneous cell sample populations, and is often used in cell biology, clinical medicine, and drug development, etc. It is especially important for the detection, diagnosis and treatment of diseases. Among the cell separation technologies and methods that use microfluidic chips as a technical platform, integrated microfluidic dielectrophoresis (DEP) chips have become a new biochemical analysis technology that researchers at home and abroad are very concerned about. At present, relevant researches only use dielectrophoresis (DEP) as the means to achieve the purpose of cell enrichment, and the chip res...

Claims

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Application Information

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IPC IPC(8): C12M1/42C12M1/00
CPCC12M47/04
Inventor 徐溢曾雪郝敦玲曹强
Owner CHONGQING UNIV
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