Method for producing 17 alpha-methyl-teslosterone by employing spherical arthrobacterium

A technology of Arthrobacter globosa and hydrogen methyl testis, which is applied in the field of microbial pharmacy and medical engineering, can solve the problems of restricting large-scale industrial production, lengthy process steps, difficult quality assurance, etc., and achieves easy separation and purification, and specific reaction Good, the effect of a single product

Inactive Publication Date: 2008-08-27
TIANJIN UNIV OF SCI & TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The process steps of the method are long, the introduction of many chemical reagents is difficult to ensure the quality, the cost is high, the feeding concentration is low, and the conversion effect is poor, which limits large-scale industrial production

Method used

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  • Method for producing 17 alpha-methyl-teslosterone by employing spherical arthrobacterium
  • Method for producing 17 alpha-methyl-teslosterone by employing spherical arthrobacterium
  • Method for producing 17 alpha-methyl-teslosterone by employing spherical arthrobacterium

Examples

Experimental program
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Effect test

Embodiment 1

[0032] A production method of 17α-dehydromethyltestosterone, the steps of the production method are:

[0033] (1). First-level seed culture: Arthrobacter sphaericus (A.Globiformis ATCC 31250) is used as the production strain to connect a full ring from the slope to 30ml seed medium, on a rotary shaker with a speed of 160r / min and a temperature of 30°C Cultivate under the conditions for 22 hours to obtain a seed culture solution suitable for inoculation.

[0034] (2). Secondary fermentation culture: insert the seed culture solution into the fermentation medium with an inoculation amount of 5% volume ratio, control the shaking table speed at 180r / min during the fermentation process, and cultivate at 32°C for 24 hours , to obtain cell fermentation liquid.

[0035] (3). Transformation: Grind 17α-methyltestosterone into fine powder, with a particle size of 14μ, and put it into the bacterial fermentation broth under aseptic conditions, and the feeding amount is 5g / L; then add hydro...

Embodiment 2

[0038] (1). Primary seed culture: Arthrobacter sphaericus (A.Globiformis ATCC 31250) is connected to a 30ml seed medium from a slant, and cultured at 32°C for 18 hours on a rotary shaker at 180r / min. Promptly obtain the seed culture solution suitable for inoculation;

[0039] (2).Secondary fermentation culture: insert the well-grown seed culture solution into 30ml fermentation medium at an inoculum size of 3% (v / v); control the shaking table speed 170r / min during the fermentation process, 30°C Cultivate for 22 hours to obtain the cell fermentation liquid;

[0040] (3). Transformation: Grind 17α-methyltestosterone into fine powder, with a particle size of 15μ, and put it into the fermentation broth of bacteria under aseptic conditions, and the feeding amount is 6g / L; then add hydrophilic to the fermentation broth of bacteria 3% ethanol as a neutral organic solvent; in C 1，2 During the dehydrogenation conversion process, the rotation speed of the shaker was controlled at 190 r...

Embodiment 3

[0043] (1). Primary seed culture: Arthrobacter sphaericus (A.Globiformis ATCC 31250) is connected to a full ring from the slope to 30ml seed medium, and cultivated at 31°C for 20 hours on a rotary shaker at 170r / min. Promptly obtain the seed culture solution suitable for inoculation;

[0044] (2). Secondary fermentation culture: insert the well-grown seed culture solution into 30ml fermentation medium at an inoculum size of 4% (v / v); control the shaking table speed 160r / min during the fermentation process, 33 Cultivate for 18 hours at ℃ to obtain the cell fermentation broth;

[0045] (3). Transformation: Grind 17α-methyltestosterone into fine powder, with a particle size of 16μ, and put it into the bacterial fermentation broth under aseptic conditions, and the feeding amount is 8g / L; then add hydrophilic to the bacterial fermentation broth Proactive organic solvent 5% dimethyl sulfoxide, in C 1，2 During the dehydrogenation conversion process, the rotation speed of the shaker...

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Abstract

The invention relates to a process which is used to produce 17 alpha-methyl-testosterone through adopting arthrobacter globiformis, which is a production process which uses arthrobacter globiformis (Arthobacter Globiformis ATCC 31250) as strain, uses 17 alpha-methyl-testosterone as substrate, and adopts one 7 alpha-methyl-testosterone (alias name: methandienone) of microbe catalytic substrate A ring inducing C1, 2-bit double bone. The process specifically comprises using arthrobacter globiformis as strain, conducting one-level seed culture and two-level fermenting culture, adding 17 alpha-methyl-testosterone dehydrogenation in thallus fermenting solution to transform into 17 alpha-methyl-testosterone, filtering after transforming, collecting filter mass, and finally preparing 17 alpha-methyl-testosterone. The production process concerned by the invention uses arthrobacter globiformis as production strain to transform substrate replacing simple arthrobacter in the current process, which has high yield and simple technique, is economical and practical, and plays extremely significant role for producing steroid medicament with biological transformation process.

Description

technical field [0001] The invention belongs to the field of microbial pharmacy and medical engineering, and specifically relates to a method for producing 17α-dehydromethyltestosterone by using Arthrobacter sphaericus. The method uses Arthrobacter sphaericus as the strain and 17α-methyltestosterone as the substrate and using microorganism C 1，2 A method for the production of 17α-dehydromethyltestosterone by dehydrogenation conversion. Background technique [0002] 17α-dehydromethyltestosterone (Dehydro-17α-methyltestosterone) is Methandienone (Methandienone), its chemical name is: 17α-methyl-17β-hydroxyandrost-1,4-dien-3-one (17β -hydroxy-17α-methylandrosta-1, 4-dien-3-one), the structural formula is shown in the figure below, it is a male hormone and anabolic hormone, compared with 17α-methyl testosterone, it has a stronger assimilation effect, male-like The effect is small, and it can also be used as a raw material intermediate for other steroid hormones. 17α-Dehydrome...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P33/02C12R1/06
Inventor 路福平杜连祥张艺王春霞李玉毛淑红
Owner TIANJIN UNIV OF SCI & TECH
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