Method of sequential extraction of biological activity component from jujube

A bioactive ingredient and graded extraction technology is applied in the field of extracting a variety of bioactive ingredients from jujube, which can solve the problems of not being able to make the best use of jujube resources and pollution of organic solutions, and achieve the effects of low cost and simple operation.

Inactive Publication Date: 2011-07-20
INST OF COMPREHENSIVE AGRI PROD UTILIZATION SHANXI PROV ACAD OF AGRI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The invention provides a method for extracting total flavonoid alcohol-soluble extract, jujube thick polysaccharide, nucleotide extract and dietary fiber from jujube, the purpose of which is to solve the organic solution pollution and jujube resources in the prior art. The problem of making the best use of everything, through graded and comprehensive extraction, the effect of extracting all the bioactive components contained in jujube has been realized

Method used

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  • Method of sequential extraction of biological activity component from jujube
  • Method of sequential extraction of biological activity component from jujube

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Remove the inedible parts such as pests and diseases from the mature jujube fruits, wash them with running tap water, and manually remove the pits. Weigh 1Kg jujube fruit and place it in a blast drying oven, and dry it at 60-65°C for 10 hours until the moisture content is about 10%. The dried jujube fruit is taken out, after cooling, mechanically crushed into 10-mesh granules.

[0024] Add 10L of 65% ethanol to the jujube fruit granules, and extract them with an ultrasonic wave with a frequency of 25KHz and a power of 200W for 40min at 50°C, repeat the extraction twice, and stand to separate. The filtrates were combined, concentrated under reduced pressure at 60°C and 0.08 MPa, and ethanol was recovered to obtain 460 g of alcohol-soluble extract, the main component of which was jujube flavonoids.

[0025] 10L of deionized water was added to the jujube dregs obtained after alcohol extraction to adjust the pH of the solution to 7. The conventional water bath method was u...

Embodiment 2

[0031] Remove the inedible parts such as pests and diseases from the mature jujube fruits, wash them with running tap water, and manually remove the pits. Weigh 500g jujube fruit and place it in a blast drying oven, and dry it at 60-65°C for 10h until the water content is about 10%. The dried jujube fruit is taken out, after cooling, mechanically crushed into 20-mesh granules.

[0032] Add 6L of 70% ethanol to the jujube granules, extract with a microwave frequency of 40MHz and power of 500W for 30min at 60°C, repeat the extraction twice, and stand for separation. The filtrates were combined, concentrated under reduced pressure at 63°C and 0.09 MPa, and ethanol was recovered to obtain 238 g of alcohol-soluble extract, the main component of which was jujube flavonoids.

[0033] Add 4.5L of deionized water to the jujube dregs obtained after alcohol extraction, adjust the pH of the solution to 5, and extract with a conventional water bath method, the extraction temperature is 50...

Embodiment 3

[0039] Remove the inedible parts such as corrosion by diseases and insect pests from the mature jujube fruit, wash them with running tap water, and remove the cores by an automatic pitting machine. Weigh 1 kg of jujube fruit and place it in a blast drying oven, and dry it at 60-65°C for 12 hours until the water content is about 8%. The dried jujube fruit is taken out, after cooling, mechanically crushed into 40-mesh granules.

[0040] Add 10L of 70% ethanol to the jujube granules, and extract in a water bath at 60°C for 2 hours. Repeat the extraction 3 times and let stand to separate. Combine the filtrates, concentrate under reduced pressure at 58°C and 0.07 MPa, and recover ethanol to obtain 505 g of alcohol-soluble extract, the main component of which is jujube flavonoids.

[0041]Add 9.2L of deionized water to the jujube dregs obtained after alcohol extraction, adjust the pH of the solution to 11, use microwave extraction, frequency 50MHz, power 800W, temperature 50°C, ti...

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Abstract

The invention discloses a fractional method for extracting bioactive components from dates; the dates are selected, enucleated, washed with water, dried, ground into particles, added with 65-75% of ethanol to be extracted for 2-3 times, and separated into date slag and filtrate; the filtrate is concentrated to obtain total flavonol soluble extract, the date slag is added with deionized water, theweight of which is 10-15 times that of the date slag, to be extracted for 2-3 times and separated into water extract and residue; the water extract is concentrated to 1 / 8-1 / 6 of the original volume, the concentrated solution is added with 95% of ethanol which is 4-6 times that of the volume of the concentrated solution to carry out alcohol precipitation separation for 10-18 hours, so as to obtainprecipitate and filtrate; the precipitate is washed by the ethanol with the concentration of over 98% for 2-3 times, and is dried to obtain date crude polysaccharide, the filtrate is concentrated to obtain ribonucleotide water soluble extract; the residue is dried and ground into dietary fiber powder. The method realizes one-step fractional extraction of a plurality of active components from the dates, has simple operation, and does not adopt any organic solvent except ethanol, thus having safety, no toxicity and residue; the active components in dates can be completely extracted, thus achieving the purpose of making best use of dates.

Description

technical field [0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting various bioactive components from jujube. The invention adopts a simultaneous graded extraction technology to extract total flavonoid alcohol-soluble extract, jujube thick polysaccharide, nucleotide extract and dietary fiber from jujube. Background technique [0002] Jujube is the ripe fruit of the perennial deciduous plant jujube of Rhamnaceae, and has been listed as one of the "five fruits" (peach, plum, plum, apricot, jujube) since ancient times, with a long history. Jujube fruit contains various bioactive components (including monosaccharides, oligosaccharides, polysaccharides, proteins, fats, organic acids, minerals, nucleotides, triterpenoids, flavonoids, etc.), and has multiple nutrients. Value and health care function, it can be regarded as a natural treasure house of nutrients. Jujube is a traditional medicinal and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23L1/28A23L1/212A23L1/30A23L1/308A23L33/10A23L33/125A23L33/13A23L33/22
Inventor 杨春卢健鸣陕方丁卫英刘金凤栗红瑜李云龙杨碧琳
Owner INST OF COMPREHENSIVE AGRI PROD UTILIZATION SHANXI PROV ACAD OF AGRI
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