Method of sequential extraction of biological activity component from jujube

Abstract

The invention discloses a fractional method for extracting bioactive components from dates; the dates are selected, enucleated, washed with water, dried, ground into particles, added with 65-75% of ethanol to be extracted for 2-3 times, and separated into date slag and filtrate; the filtrate is concentrated to obtain total flavonol soluble extract, the date slag is added with deionized water, theweight of which is 10-15 times that of the date slag, to be extracted for 2-3 times and separated into water extract and residue; the water extract is concentrated to 1 / 8-1 / 6 of the original volume, the concentrated solution is added with 95% of ethanol which is 4-6 times that of the volume of the concentrated solution to carry out alcohol precipitation separation for 10-18 hours, so as to obtainprecipitate and filtrate; the precipitate is washed by the ethanol with the concentration of over 98% for 2-3 times, and is dried to obtain date crude polysaccharide, the filtrate is concentrated to obtain ribonucleotide water soluble extract; the residue is dried and ground into dietary fiber powder. The method realizes one-step fractional extraction of a plurality of active components from the dates, has simple operation, and does not adopt any organic solvent except ethanol, thus having safety, no toxicity and residue; the active components in dates can be completely extracted, thus achieving the purpose of making best use of dates.

Description

technical field

[0001] The invention belongs to the technical field of natural product extraction, and in particular relates to a method for extracting various bioactive components from jujube. The invention adopts a simultaneous graded extraction technology to extract total flavonoid alcohol-soluble extract, jujube thick polysaccharide, nucleotide extract and dietary fiber from jujube. Background technique

[0002] Jujube is the ripe fruit of the perennial deciduous plant jujube of Rhamnaceae, and has been listed as one of the "five fruits" (peach, plum, plum, apricot, jujube) since ancient times, with a long history. Jujube fruit contains various bioactive components (including monosaccharides, oligosaccharides, polysaccharides, proteins, fats, organic acids, minerals, nucleotides, triterpenoids, flavonoids, etc.), and has multiple nutrients. Value and health care function, it can be regarded as a natural treasure house of nutrients. Jujube is a traditional medicinal and...

Images