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Method for preparing microorganism with xylem filber raw material

A microbial oil and wood fiber technology, applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of restricting the industrialization process of biodiesel, high cost, and difficulty in collecting and transporting raw materials.

Inactive Publication Date: 2008-10-29
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the research on biodiesel preparation at home and abroad mainly focuses on vegetable oil or waste catering oil, but the cost of using vegetable oil as raw material is high, and its cost accounts for 70% to 85% of the total production cost. The difficulty of collecting and transporting raw materials has seriously restricted the industrialization process of biodiesel, so finding a cheap raw material has become the key to the industrialization of biodiesel

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0019] 1. Bagasse pretreatment

[0020] Take bagasse with a certain weight of 20-40 mesh and dry constant weight in a conical flask, first use 1% (mass fraction) NaOH to treat the bagasse on a shaker at a liquid-solid ratio of 25:1 for 20 hours, and keep the temperature constant. The temperature is 30°C, and the oscillator speed is 140r / min. After the pretreatment, wash with deionized water and filter with suction, dry the bagasse that has been washed to neutral at 105°C for 4 hours in a blast drying oven, cool to room temperature in a desiccator, and store it in a sample bag for future use. The dried bagasse is subjected to dilute acid hydrolysis. Sulfuric acid concentration = 1.24%, time = 2.64h, temperature = 123°C, liquid-solid ratio fixed at 10:1. The obtained hydrolyzate was distilled under reduced pressure to remove furfural, and the hydrolyzate was concentrated until the total sugar concentration was about 30 g / L, the pH of the hydrolyzate was adjusted to 6 with KOH,...

Embodiment 2

[0033] 1. Bagasse pretreatment

[0034] Take bagasse with a certain weight of 20-40 mesh and dry constant weight in a conical flask, first use 1% (mass fraction) NaOH to treat the bagasse on a shaker at a liquid-solid ratio of 25:1 for 20 hours, and keep the temperature constant. The temperature is 30°C, and the oscillator speed is 140r / min. After the pretreatment, wash with deionized water and filter with suction, dry the bagasse that has been washed to neutral at 105°C for 4 hours in a blast drying oven, cool to room temperature in a desiccator, and store it in a sample bag for future use. The dried bagasse is subjected to dilute acid hydrolysis. Sulfuric acid concentration = 1.24%, time = 2.64h, temperature = 123°C, liquid-solid ratio fixed at 10:1. The obtained hydrolyzate was distilled under reduced pressure to remove furfural, and the hydrolyzate was concentrated until the total sugar concentration was about 50 g / L, the pH of the hydrolyzate was adjusted to 6 with KOH,...

Embodiment 3

[0047] 1. Corn cob pretreatment

[0048] Take a certain weight of 20-40 mesh corn cob raw materials with constant weight in the Erlenmeyer flask, first use 1% (mass fraction) NaOH to treat the bagasse on a shaker at a liquid-solid ratio of 25:1 for 20 hours, the temperature The temperature is fixed at 30°C, and the oscillator speed is 140r / min. After the pretreatment, wash with deionized water and filter with suction, dry the corn cob raw material washed to neutral at 105°C for 4 hours in a forced air drying oven, cool to room temperature in a desiccator, and store it in a sample bag for future use. The dried bagasse is subjected to dilute acid hydrolysis. Sulfuric acid concentration = 1.15%, time = 1h, temperature = 115°C, liquid-solid ratio fixed at 10:1. The obtained hydrolyzate was distilled under reduced pressure to remove furfural, and the hydrolyzate was concentrated until the total sugar concentration was about 50 g / L, and the pH of the hydrolyzate was adjusted to 6 ...

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Abstract

A method for preparing microbial oils from wood fiber materials, which belongs to the field of biochemical technology, comprises the following steps: preparing hydrolysate from wood fiber materials by two hydrolysis steps, and preparing the microbial oils from the hydrolysate by two material feeding and fermenting processes. The method can prepare microbial oils with high output and content from the wood fiber materials. The fermentation substrate and the feeding substrate can be selected from the hydrolysate of the wood fiber materials, thus sufficiently utilizing the wood fiber resources and lowering the production cost.

Description

technical field [0001] The invention belongs to the field of biochemical technology, in particular to a method for preparing microbial oil from lignocellulosic raw materials. Background technique [0002] Biodiesel is a renewable new energy developed in recent years based on environmental degradation and energy crisis that can replace fossil fuels. Biodiesel is a renewable clean energy with great development potential. At present, the research on biodiesel preparation at home and abroad mainly focuses on vegetable oil or waste catering oil, but the cost of using vegetable oil as raw material is high, and its cost accounts for 70% to 85% of the total production cost. The difficulty of collecting and transporting raw materials has seriously restricted the industrialization process of biodiesel, so finding a cheap raw material has become the key to the industrialization of biodiesel. [0003] Microbial oil, also known as single cell oil, is made of microorganisms such as yeast...

Claims

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Application Information

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IPC IPC(8): C12P7/64C12N1/16C12N1/14C11B1/04C12R1/645
Inventor 刘宏娟张建安武敏敏李建程可可周玉杰
Owner TSINGHUA UNIV