Nest alkane type diterpenoid and abstraction and separation method thereof

A separation method and compound technology, which is applied in the separation/purification of carboxylic acid esters, organic chemistry, medical raw materials derived from fungi, etc., can solve the problems such as no reports of bird's nest alkane-type diterpene compounds, and achieve cost reduction and Difficulty of operation, simple and feasible method

Inactive Publication Date: 2008-11-19
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, among the many reports retrieved, there is no report about the ornithine alkane type diterpene compound I and II involved in the present invention

Method used

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  • Nest alkane type diterpenoid and abstraction and separation method thereof
  • Nest alkane type diterpenoid and abstraction and separation method thereof
  • Nest alkane type diterpenoid and abstraction and separation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Soak 1 kg of dried fruiting bodies of C. crassa in methanol at room temperature for 36 hours, filter, recover methanol to obtain 65 g of extract. The extract was partitioned and extracted with chloroform-water, V (chloroform): V (water) = 6: 4, the solvent was concentrated and recovered to obtain 37 g of chloroform, and the aqueous phase was dissolved with methanol after concentration to obtain 22 g of methanol.

[0020] The chloroform part was transferred to a silica gel column, followed by V (petroleum ether): V (acetone) = 9: 1, V (petroleum ether): V (acetone) = 8: 2 eluent elution to remove impurities, and then use V (petroleum ether): V (acetone) = 7: 3 eluent eluted, collected eluent, recovered solvent.

[0021] The eluate obtained in the above steps was subjected to RP-8 column chromatography twice, and was eluted with the eluent of V (methanol): V (water) = 9: 1, and then through Sephadex LH-20, with 1: 1 Chloroform / methanol eluted and separated to obtain 10 m...

Embodiment 2

[0023] Soak 2kg of dry fruiting bodies of C. crassa in methanol at room temperature for 50 hours, filter, recover methanol to obtain 120g of extract. The extract was partitioned and extracted with chloroform-water, V (chloroform): V (water) = 5.5: 4.5, the solvent was concentrated and recovered to obtain 70 g of the chloroform portion, and the aqueous phase was dissolved with methanol to obtain 40 g of the methanol portion after concentration.

[0024] The chloroform part was transferred to a silica gel column, followed by V (petroleum ether): V (acetone) = 9: 1, V (petroleum ether): V (acetone) = 8: 2 eluent elution to remove impurities, and then use V (petroleum ether): V (acetone) = 7: 3 eluent eluted, collected eluent, recovered solvent.

[0025] The eluate obtained in the above steps was subjected to 3 times of RP-8 column chromatography, eluted with the eluent of V (methanol): V (water) = 8: 2, and then through Sephadex LH-20, with 1: 1 Chloroform / methanol eluted and se...

Embodiment 3

[0027] Soak 3kg of dried fruiting bodies of C. crassa in methanol at room temperature for 55 hours, filter, recover methanol to obtain 190g of extract. The extract was extracted with chloroform-water distribution, V (chloroform): V (water) = 5: 5, the solvent was concentrated and recovered to obtain 110 g of chloroform, and the aqueous phase was dissolved with hot methanol after concentration to obtain 68 g of methanol.

[0028] The chloroform part was transferred to a silica gel column, followed by V (petroleum ether): V (acetone) = 9: 1, V (petroleum ether): V (acetone) = 8: 2 eluent elution to remove impurities, and then use V (petroleum ether): V (acetone) = 7: 3 eluent eluted, collected eluent, recovered solvent.

[0029] The eluent obtained in the above steps was eluted with the eluent of V (methanol): V (water) = 7: 3 through 4 times of RP-8 column chromatography and RP-8 chromatography, and then subjected to Sephadex LH- 20, separated by elution with 1:1 chloroform / me...

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Abstract

The invention discloses a nest alkyl type diterpenoid compound and a method for extracting and separating the same. The compound has a structural formula I or II; an extraction and separation method comprises the following steps of raw material pulverization, leaching, extraction and column chromatogram separation. The nest alkyl type diterpenoid compound provided by the invention has good exploitation, utilization and development prospect. The extraction and separation method is simple and feasible and reduces the cost and operating difficulty of the extraction and separation method.

Description

technical field [0001] The invention relates to a diterpene compound, in particular to a bird's nest alkane type diterpene compound and an extraction and separation method thereof. Background technique [0002] Cyathane-type diterpenoids have a very regular five-membered, six-membered and seven-membered ring-connected skeleton. According to literature research, they are mainly distributed in the fungi of the family Odontomycetes and the family Aviaceae, such as the bird's nest fungus (Cyathushelence) fruiting body and fermentation liquid, Hericium erinaceum (Hericium erinaceum) fermentation liquid, fruiting body of Sarcodon scabrosus (Sarcodon scabrosus). From the discovery of the first cyathane-type diterpenes in 1973 to December 2006, the research on cyathane-type diterpenes showed that these diterpene compounds showed a wide range of physiological activities such as stimulating nerve growth factor (NGF) synthesis, antibacterial, and anti-inflammatory . (T.Ohta, T.Kita, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C69/78C07C67/48A61K36/06
Inventor 麻兵继
Owner HENAN AGRICULTURAL UNIVERSITY
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