Microbe auto culturing system using nano-sensor

Abstract

The invention belongs to the nano technology and biomedicine technical field, and particularly relates to a microorganism automatic cultivation system adopting a nano sensor. The system mainly comprises a specially designed culture flask, a nano sensor detector and a device for control, display and record. A separation film in the culture flask separates culture solution from pure water or air, and only CO2 can pass through the separation film. After a specimen is inoculated, the culture flask can carry out automatic temperature regulation and shake culture, and, at the same time, can monitor the generation status of CO2 in the flask through detecting the potential difference between electrodes formed by a nano film or a nano wire (rod) array, a metal oxide nano wire (rod) array and a counter electrode arranged on the side of the pure water or air. An obtained signal is transmitted into a computer for analysis and is judged as a positive result once the signal exceeds a preset threshold value, and the computer can automatically give an alarm. The system which can replace the prior automatic cultivation system in current use can improve a positive detectable rate with high sensitivity and simple operation.

Description

technical field [0001] The invention belongs to the technical fields of nanotechnology and biomedicine, and in particular relates to an automatic culture system for microorganisms using nanometer sensors. Background technique [0002] Pathogenic microorganisms invade the blood circulation, grow and multiply in the blood, and produce toxins and other metabolites, which can cause acute or subacute systemic infection, and can lead to septic shock and multiple organ failure. Treatment will endanger the life of the patient. Blood culture examination is of great significance for the diagnosis of blood and circulatory system infections such as bacteremia, sepsis and bacterial endocarditis. [0003] The early blood culture system was mainly a manual method. Although the cost was low, it required operators to have certain experience, which consumed a lot of manpower and time, and the culture medium used was often prepared by each laboratory, so it was difficult to achieve standardiz...

AI Technical Summary

Problems solved by technology

This detection system has not been widely used due to its relatively low sensitivity

[0009] The blood culture instruments of the above detection methods all have false negatives in actual clinical work, that is, when the instrument does not alarm, manual blind transmission can be found to have bacterial growth. The reason may be due to the slow growth of bacteria. , the released CO 2 The amount is not enough to be detected by the instrument, that is, the detection sensitivity is too low

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