Palm shaped aspidiaceae spore breeding method
A technology for spores and fork ferns, which is applied in the field of spore reproduction of palmate fork ferns, can solve the problems of low survival rate of natural reproductive cycle and few propagation methods of palmate fork ferns, etc., and achieves the effect of shortening the breeding cycle.
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Embodiment 1
[0014] Cut the mature sporophylls of Pteridocarpus palmatum and place them on dry filter paper, collect 0.03 g of spores, wrap them with filter paper, and use gibberellin (GA) with a concentration of 10 mg / L 3 ) water solution 10mL soak for 5min.
[0015] Mix the flower soil, plain sand and vegetable garden soil at a volume ratio of 2:1:1 to obtain a soil substrate with a pH of 6.3 to 6.4, sow the spores of Forglena palmatum soaked in gibberellin on the soil substrate, and cultivate at a temperature of 25 to 6.4. 30°C, the light intensity is 2 000-3 000lx, the light time is 11-13h / d, and the humidity is 60%-80%. 20 days after sowing, the spores germinated one after another. After 40 days, the gametophytes developed into lamellar to heart-shaped gametophytes, and after 70 days, the gametophytes developed into mature heart-shaped gametophytes. After 110 days, the gametophytes developed well, and a large number of young sporophytes formed.
[0016] Vegetable garden soil and flow...
Embodiment 2
[0018] Cut the mature sporophylls of Pteridocarpus palmatum and place them on dry filter paper, collect 0.03 g of spores and wrap them with filter paper, and use gibberellin (GA) with a concentration of 50 mg / L 3 ) water solution 10mL soak for 4min.
[0019] Mix the flower soil, plain sand and vegetable garden soil at a volume ratio of 2:1:1 to obtain a soil substrate with a pH of 6.3 to 6.4, sow the spores of Forglena palmatum soaked in gibberellin on the soil substrate, and cultivate at a temperature of 25 to 6.4. 30°C, the light intensity is 2000-3000lx, the light is 11-13h / d, and the humidity is 60%-80%. 20 days after sowing, the spores germinated one after another. After 40 days, the gametophytes developed into lamellar to heart-shaped gametophytes, and after 70 days, the gametophytes developed into mature heart-shaped gametophytes. After 110 days, the gametophytes developed well, and a large number of young sporophytes formed.
[0020] Vegetable garden soil and flower s...
Embodiment 3
[0022] Cut off the mature sporophyll of Pteridocarpus palmatum and place it on dry filter paper, collect 0.03g of spores, wrap the collected spores with filter paper, and use gibberellin (GA) with a concentration of 75mg / L 3 ) water solution 10m soak for about 6min.
[0023] Mix the flower soil, plain sand and vegetable garden soil at a volume ratio of 2:1:1 to obtain a soil substrate with a pH of 6.3 to 6.4, sow the spores of Forglena palmatum soaked in gibberellin on the soil substrate, and cultivate at a temperature of 25 to 6.4. 30°C, the light intensity is 2000-3000lx, the light is 11-13h / d, and the humidity is 60%-80%. 20 days after sowing, the spores germinated one after another. After 40 days, the gametophytes developed into lamellar to heart-shaped gametophytes, and after 70 days, the gametophytes developed into mature heart-shaped gametophytes. After 110 days, the gametophytes developed well, and a large number of young sporophytes formed.
[0024] Vegetable garden ...
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