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Hydroxy fatty acid oligomer and application in regulating calcium ion concentration in cell

A cell and oligomerization technology, applied in the direction of drug combination, metabolic disease, cardiovascular system disease, etc., can solve the problem of high price

Inactive Publication Date: 2008-12-03
SHANTOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But most of them are only used in the laboratory stage at present, and the price is expensive (Liu Daoming, 2000)

Method used

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  • Hydroxy fatty acid oligomer and application in regulating calcium ion concentration in cell
  • Hydroxy fatty acid oligomer and application in regulating calcium ion concentration in cell
  • Hydroxy fatty acid oligomer and application in regulating calcium ion concentration in cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0054] The preparation of embodiment 1 3-hydroxybutyric acid oligomer (OHB)

[0055] Accurately weigh 3 g of poly-3-hydroxybutyrate-4-hydroxybutyrate (P3HB4HB), add 200 ml of dichloromethane, and reflux at 90°C. After the PHB is completely dissolved, add 0.3 g of p-toluenesulfonic acid and 0.5 g of water, continue the reflux reaction for 1 hour, and then add 0.5 g of water. After 2 hours, the reaction was terminated, filtered, precipitated with cold methanol, filtered, extracted by a Soxhlet extractor for 12 hours, and then dried under vacuum at 50°C for 48 hours for later use. The number-average molecular weight (number-average molecular weight or Mn) detected by gel permeation chromatography (GPC, Waters1515) is 2000, and the nuclear magnetic resonance spectrum (NMR, Bruker 400) analysis product does not contain cytotoxic substances such as ethylenic bonds.

Embodiment 2

[0056] Example 2 Preparation of oligomeric 3-hydroxybutyrate methyl ester (OHB-CH3)

[0057] Dissolve 1 g of PHB in 50 mL of chloroform, heat to reflux at 60°C for 2 hours to fully dissolve, add dropwise 50 mL of concentrated sulfuric acid methanol solution (10% v / v), and heat to reflux at 90°C for 2 hours. Add 50mL of methanol pre-cooled at 4°C, let stand for 1h, centrifuge the mixed solution at 8000rpm, 4°C for 20min, discard the supernatant, add methanol to resuspend. Repeat the above steps twice, dry under vacuum at 50°C for 48 hours for later use, and obtain OHB-OCH containing 3 Solid white powder. Gel permeation chromatography (GPC, Waters 1515) detected that the Mn was 2000, and nuclear magnetic resonance spectroscopy (NMR, Bruker 400) analyzed that the product did not contain cytotoxic substances such as ethylenic bonds.

Embodiment 3

[0058] Example 3 Oligomeric 3-hydroxybutyrate-4-hydroxybutyrate methyl ester (O3HB4HB-CH 3 ) preparation

[0059] Dissolve 1g of P3HB4HB in 50mL of chloroform, heat to reflux at 60°C for 2h to fully dissolve, add 50mL concentrated sulfuric acid methanol solution (10% v / v) dropwise, and heat to reflux at 90°C for 2h. Add 50mL of methanol pre-cooled at 4°C, let stand for 1h, centrifuge the mixed solution at 8000rpm, 4°C for 20min, discard the supernatant, add methanol to resuspend. Repeat the above steps twice, dry under vacuum at 50°C for 48 hours for later use, and obtain OHB-OCH containing 3 Solid white powder. The Mn detected by gel permeation chromatography (GPC, Waters 1515) is 2100, and the nuclear magnetic resonance spectrum (NMR, Bruker 400) analyzes that the product does not contain cytotoxic substances such as ethylenic bonds.

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Abstract

The invention provides a hydroxy fatty acid oligomer and an application of the hydroxy fatty acid oligomer in adjusting the growth of cells and improving the concentration of calcium ions in the cells. As proved by experiments, the hydroxy fatty acid oligomer can inhibit the proliferation of cancer cells, induce the apoptosis and the death of the cells, prolong the G1 period of the cells and reduce the segmentation degree of the cancer cells. As shown by the further calcium imaging technical detection, the oligomer also can stimulate the increase of the concentration of dissociative calcium ions in the cells.

Description

technical field [0001] The present invention relates to hydroxy fatty acid oligomer and its use in regulating intracellular calcium ion concentration and regulating cell growth. Background technique [0002] Overview of the Physiological Functions of Calcium Ions [0003] In the human body, the calcium element content exceeds 1000 grams, and 99% of the calcium in the human body is deposited in the bones and teeth to promote their growth and maintain their shape and hardness; the remaining less than 10 grams exist in blood and soft tissue cells It is precisely this small amount of calcium that plays a vital role in the life process. At the end of the 19th century, British cardiac physiologist Sydney Ringer (Sydney Ringer) realized that calcium It is a special factor that can maintain heart function, which opened the prelude to the study of calcium ions (Sydney, 1884). By the 1960s, the mechanism by which calcium ions drive muscle contraction was basically elucidated. With...

Claims

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Application Information

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IPC IPC(8): A61K31/25A61P3/14A61P9/04
Inventor 陈国强代忠伟孙杰
Owner SHANTOU UNIV
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