ELISA reagent kit for on-site detection

Abstract

The invention discloses an ELISA kit used for on-site detection. The kit comprises a kit body; an antibody detection plate, a sample dilution reagent, positive / negative contrast, a cleaning reagent, an enzyme conjugate reagent, a substrate reagent, a stopping reagent, a disposable dropper and a cover plate membrane are arranged in the kit body; the substrate reagent is composed of a substrate, a substrate diluent and an indicator II; the substrate diluent is a concentrated solution; the sample dilution reagent, the cleaning reagent, the enzyme conjugate reagent, the stopping reagent, the substrate and the indicator II are all solid. The ELISA kit has the advantages of great portability, convenient use and high safety. Furthermore, the requirement of the on-site detection can be met.

Description

technical field [0001] The invention relates to an ELISA kit for on-site detection. Background technique [0002] Enzyme-linked immunosorbent assay (ELISA) is a detection technique that combines the catalytic reaction of enzymes with the immunological reaction of antigens and antibodies. Due to the high specificity of the antigen and antibody reactions, coupled with the high catalytic efficiency of the enzyme, the result of the immune reaction is indirectly amplified, so the determination method can achieve high specificity and sensitivity. In recent years, ELISA has been widely used in the detection of viruses, bacteria, hormones, and drug residues due to its sensitivity, accuracy, good repeatability, and no radioactive contamination. [0003] However, the currently used ELISA kits need to be refrigerated and stored in the refrigerator because the reagents such as antibodies and enzyme markers are liquid, and cooling measures such as ice packs and incubators are also requi...

AI Technical Summary

Problems solved by technology

[0005] 1. Because the kit contains various liquid reagents such as antibody solution, enzyme marker solution, sample dilution buffer (concentrate), etc., the kit needs to be refrigerated, which makes it inconvenient to carry and mail; and the kit is in use. The titers of antibodies and enzymes are susceptible to being affected by repeated rewarming, and losses and contamination are easily caused during the process of absorbing solutions for many times. Sometimes, all kits will become invalid due to power failure or refrigerator failure.

[0006] 2. Poor biological safety: the quality control samples of the commonly used plant virus detection kits are obtained by directly freeze-drying the extracts of positive or negative samples

Although the virus is easily inactivated under dry conditions at room temperature, if it is frozen and then vacuum-dried, the virus can survive for a long time

[0007] 3. ELISA is a test with very fine operation requirements. At present, the alkaline phosphatase-labeled ELISA reagent lacks a suitable indicator, and the operation process is prone to errors, especially in the TSA-ELISA (three-antibody sandwich type) kit. Detection antibodies and enzyme-labeled anti-antibodies are two colorless and trace precious reagents. If they are not marked clearly, mistakes are more likely to occur.

[0008] 4. The currently commonly used 3mol / L sodium hydroxide terminates the alkaline phosphatase reaction, and the result can only be maintained for several hours, and sodium hydroxide is corrosive