Method for artificially collecting and cultivating larva of intertidal belt sponge with embryo

Abstract

The invention relates to a method for artificially collecting and cultivating sexual reproduction larvae of intertidal zone membrane sponge under laboratory conditions. Sponges containing mature embryo are brought back to a laboratory after being collected in an intertidal zone, fixed on a carrier after cleaned by seawater, put in an incubator for cultivation, and fed with bait algae as main food. Maternal sponges can release larvae in an artificially created environment; the larvae are attached on a selective attaching base after a planktonic period and can be grown into small sponges under the conditions of proper food and temperature. The invention provides a new technology for the seed source problem of artificially cultivated sponges and provides a new way for resolving the problem of insufficient supply of drug sources in the development of sponge drugs, thereby having broad prospect of application.

Description

technical field [0001] The present invention relates to marine organisms, and is a technique for artificially inducing sponges containing embryos to release sponge larvae in a laboratory, collecting and cultivating larvae, and specifically establishing a method for artificially collecting and cultivating sponge larvae in a laboratory. A method for artificially collecting and cultivating intertidal membranous sponge larvae. Background technique [0002] Sponges are considered to be the most potential drug source organisms in the ocean. The secondary metabolites extracted from sponges have anti-tumor, anti-fungal, and even anti-HIV activities. Therefore, the development of sponge drugs has become a hot spot in the development of marine drugs in recent years. However, during the development of sponge drugs, the bottleneck of insufficient supply of drug sources is encountered, that is, there is not enough sponge to extract a certain amount of active substances for further drug r...

AI Technical Summary

Problems solved by technology

However, during the development of sponge drugs, the bottleneck of insufficient supply of drug sources is encountered, that is, there is not enough sponge to extract a certain amount of active substances for in-depth drug research, making some drugs only stay in the clinical trial stage and cannot continue

People try to solve this problem with many methods, but all of them have key technologies that are difficult to solve, such as sponge cell culture technology. At present, there is no cell line that can be continuously passaged; chemical synthesis is expensive, the steps are complicated, and the practical feasibility is too low. ; At present, it is generally believed that sponge factory culture is considered to be one of the more promising ways to solve the problem of "insufficient drug source supply"

However, industrial farming is still in the laboratory stage, the biomass proliferation is small, and it cannot achieve continuous subculture of sponge explants under artificial controllable conditions. The source of culture always needs sponges from the ocean, that is, collecting wild natural sea areas Artificial cultivation of sponge explants, continuous collection will cause the depletion of sponge resources and the destruction of the ecological environment

The sexual reproduction of marine invertebrates is the most ideal way to increase the number of individuals rapidly. Under wild conditions, the survival rate of larvae is very low due to predation and environmental conditions.

[0003] At present, there is no research on sponge larvae in China. There are many studies abroad on sponge larvae, but most of them are about larval morphology, behavior, histology, and ecology. There are also a few reports on the collection and cultivation of larvae in the laboratory. The survival rate of larvae attachment is low, and there is no specific data on growth. There is no report on the systematic method of manual collection and cultivation of sponge larvae

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