Method for yeast cell to biosynthesizing 1,6- fructose sodium diphosphate

Abstract

The invention discloses a method for biologically synthesizing 1, 6-di- (2- ethylhexyl ) phosphoric acid fructose sodium of a yeast cell, comprising the following steps that: by taking 100mL of sterilizing reaction fluid to count, 1 to 5 grams of thalli which is obtained by pre-culture process is added to react for 4 to 10 hours at a temperature of between 25 and 30 DEG C and at a speed of 100 to 200r / min, trichloroacetic acid is added to stop the reaction, the clear solution of the 1, 6-di- (2- ethylhexyl ) phosphoric acid fructose sodium is obtained by centrifugalization; the thalli is cells which are obtained by pre-culturing for 12 to 36 hours, freezing and centrifuging of Saccharomyces cerevisiae; the pH value of the reaction fluid is between 5.0 and 9.0, the compositions of the reaction fluid in weight percentage are: 2 to 12 percent of glucose, 2 to 15 percent of sodium phosphate dibasic and the balance being water. The 1, 6-di- (2- ethylhexyl ) phosphoric acid fructose sodium synthesized by the method has simple process for culturing the yeast cells, shortens the time, and has simple converting system, easy operation and high FDP sodium converting efficiency.

Description

technical field

[0001] The invention belongs to the fields of bioengineering and microbial fermentation, and in particular relates to a method for yeast cell biosynthesis of sodium fructose 1,6-diphosphate. Background technique

[0002] 1,6-diphosphate fructose [Fructose-1,6-Diphosphate, referred to as FDP], the molecular formula is C 6 h 14 o 12 P 2 , often in the form of sodium, calcium, zinc and other salts. It is an intermediate product of sugar metabolism, soluble in water, necessary for human life activities, and plays an important role as an attenuator, biocatalyst and cell strengthening agent in the process of cell metabolism.

[0003] Since Harden and Young discovered and clarified the basic characteristics of 1,6-diphosphate fructose in 1908, many scientists have studied the preparation and application of 1,6-diphosphate fructose. People such as Nenfeng synthesized FDP in the laboratory with the enzyme system of fresh baker's yeast or brewer's yeast in 1942, w...