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Method for separating and purifying total flavonoids

A technology for separation and purification of total flavonoids, which can be applied to medical preparations containing active ingredients, pharmaceutical formulations, plant/algae/fungus/moss components, etc., and can solve the problems of poor purification effect, small adsorption capacity of flavonoids, and low desorption rate. , to achieve the effect of easy desorption regeneration, high adsorption capacity and large adsorption capacity

Inactive Publication Date: 2008-12-31
ZHEJIANG GONGSHANG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional macroporous adsorption resins have small adsorption capacity (50mg / g-80mg / g) for flavonoids, low desorption rate (70%-80%), and poor purification effect (the total flavonoid content of the final product is about 40%)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Synthesis of Example 1

[0036] Synthesis of Special Adsorption Resin for Separation and Purification of Jumi

[0037] Add 1000ml of distilled water, 10ml of gelatin, 200g of sodium chloride, etc. into a 2000ml three-necked bottle in sequence. Ethylene (St) 10g, allyl methacrylate (AMA) 10g, dimethylaminoethyl methacrylate (DMAEMA) 5g, porogen toluene (PhMe) 110g, 200# gasoline 40g and initiator azobis 252g of the oil phase prepared from 2g of isobutyronitrile (AIBN) etc. was slowly added into the reactor. Control the stirring intensity so that the particle size of the monomer droplets is in the range of 0.2-1.0mm, raise the reaction temperature of the reactor to 70-75°C, and keep the temperature until the beads are shaped; raise the reaction temperature to 80-85°C and keep warm 5 hours, heat preservation at 85-90°C for 5 hours; lower the temperature of the reactor to 30-40°C, filter out the beads, and wash with a large amount of water; transfer the washed beads to a ...

Embodiment 2

[0038] Example 2 Adsorption

[0039] Fill a φ55×800mm glass adsorption column with 700ml of special macroporous adsorption resin. The ratio of the height to diameter of the resin bed in the adsorption column is in the range of 5-13. Flow through the adsorption column, the room temperature is ≤35°C, the measured flow rate is 1-2BV per hour, and the concentration of total flavonoids is regularly sampled and analyzed until the concentration of the original solution is reached, then the adsorption is stopped

Embodiment 3

[0040] Example 3 Adsorption

[0041] Fill 700ml of special macroporous adsorption resin in the glass adsorption column of φ55×800mm, the ratio of height to diameter of the resin bed in the adsorption column is in the range of 5-13, and the concentration of total flavonoids in the first purified liquid after filtration is adjusted to 3.5mg / mL, flow through the adsorption column from top to bottom, control the flow rate of waste water to 1-2BV per hour according to the volume of the resin bed, and regularly sample and analyze until the total brass concentration reaches the concentration of the sample solution.

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PUM

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Abstract

The invention relates to a method for separating and purifying total flavonoids from jumi. The special macroporous absorption resin for separating and purifying total flavonoids is adopted to dynamically absorb the total flavonoids in the jumi, after the absorption is saturated, dynamic desorption and distillation are adopted to separate and purify the total flavonoids; after the first-time purification, the sample solution is purified for the second time in the same way; the height to the diameter of a resin bed in an absorption column ranges from 5 to 13, the pH of extract is adjusted to be 5.0-6.0, the room temperature is controlled to be less than or equal to 35 DEG C, the flow rate of the extract is controlled to be 1-2BV per hour, 70% ethanol is adopted to conduct desorption, the desorption solution is distilled, and the total flavonoids is obtained after the ethanol is removed. Due to the special macroporous absorption resin absorbs total flavonoids in jumi with high efficiency, the content of total flavonoids in the product exceeds 85%.

Description

technical field [0001] The invention belongs to the field of separation and purification of natural products, and relates to a separation and purification process of effective substances in natural products, in particular to a method for separating and purifying total flavonoids from Jumi. Background technique [0002] Chrysanthemum is made from flower buds of wild chrysanthemum, which is dried and dried. Flavonoids are one of the main active ingredients of wild chrysanthemum (the total flavonoid content of the primary extract of chrysanthemum can reach 13%). At present, the research on chrysanthemum still stays at the level of cultivation technology and pharmacological effect, and there is no report on the separation and purification of the medicinal components of chrysanthemum. [0003] At present, traditional macroporous adsorption resins (AB-8, D101, etc.) are mostly used in the purification of total flavonoids of natural products in China. Traditional adsorption resins ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/28A61K133/00
Inventor 熊春华姚彩萍何崎吴香梅
Owner ZHEJIANG GONGSHANG UNIVERSITY
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