Non-blood serum low density culture medium for animal cell and use thereof

Abstract

The invention discloses a culturing medium for cultivating animal cells without serum and with low density and an application thereof. The culturing medium for cultivating animal cells without serum and with low density is characterized in that synthetic culturing medium is adopted as the raw material, and insulin powder, transferrin powder, L-glutamine powder, sodium selenite powder, ethanolamine solution, lipid solution, epidermal growth factor powder, a recombinant human acidic fibroblast growth factor, vitamin solution, plant hydrolysate solution, putrescine, ferrous sulfate, hydrocortisone and an insulin-like factor are added into the synthetic culturing medium. The culturing medium can be suitable for the growth and metabolism and product expression of the cells of the mammals in the condition of low density without the serum. Combined with the method that D- polylysine powder is coated on a culture plate with 96 holes, the use of the culturing medium for cultivating animal cells without serum and with low density can clone the animal cells without the serum, thereby realizing the continuity of cultivating the animal cells without the serum, improving the stability of product expression, assuring the quality and safety of the product, and enhancing the controllability of the production process. Thus, the culturing medium for cultivating animal cells without serum and with low density has wide application range.

Description

technical field [0001] The invention belongs to the cell engineering technology in the field of biopharmaceuticals, and mainly relates to animal cell serum-free low density (50-5×10 3 cells / mL) medium and its application. The animal cell serum-free low-density medium can not only carry out the serum-free cloning screening of various animal cells but also meet the density of 50-5×10 3 Cell growth metabolism and product expression at cells / mL. Background technique [0002] Animal cell culture is one of the key technologies widely used in the basic theoretical research of life sciences and in the field of biotechnology. Animal cells have been widely used in the biopharmaceutical industry due to their high activity and good stability. At present, 70% of the biotechnology drugs approved by the US FDA are expressed and produced by animal cells. The widely used animal cells are CHO, S p 2 / 0, HEK293, Vero and BHK etc. [0003] In the biopharmaceutical industry, optimizing and ...

AI Technical Summary

Problems solved by technology

Adopting the present invention makes it possible to establish a high-efficiency and stable expression platform for animal cells for production; in the production process, cells beyond the stationary phase can be screened by the present invention to obtain cell strains with stable growth metabolism and product expression; cell strain transformation and cell line optimization become possible; it can be used as a new method for quality control detection of cell banks at the end of production, and solves the technical bottleneck in the field of biopharmaceuticals

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