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Porous microspheres and use thereof in preparing porous microspheres vaccine

A technology of porous microspheres and microspheres, which can be used in medical preparations of non-active ingredients, bacterial antigen components, antibacterial drugs, etc., and can solve problems such as loss of activity

Inactive Publication Date: 2009-02-11
MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the preparation process, the antigenic protein is exposed to environments such as organic solvents, high mechanical shear force, aqueous phase-organic phase interface, and high temperature, and the activity tends to be greatly lost.

Method used

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  • Porous microspheres and use thereof in preparing porous microspheres vaccine
  • Porous microspheres and use thereof in preparing porous microspheres vaccine
  • Porous microspheres and use thereof in preparing porous microspheres vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1, preparation of PLGA porous microsphere vaccine containing V-antigen protein and its immune protection evaluation

[0025] 1. Preparation of PLGA porous microsphere vaccine

[0026] 1. Preparation of PLGA porous microspheres

[0027] Dissolve 500mg of PLGA in 2mL of a mixture of acetone and dichloromethane (acetone-dichloromethane 1:1), then add this mixture to 200μl saturated sodium chloride solution, shake at 9000rpm for 15s to make colostrum; Cool the colostrum in an ice-water bath for 2 minutes, add 10 mL of 1% (0.1-2%) PVA solution containing 5% (1-10%) sodium chloride in a mass / volume ratio, shake at 8000rpm for 15s to make a compound Milk; then transfer the double milk to another 90mL of 1% (0.1-2%) PVA solution containing 5% (1-10%) sodium chloride in a mass / volume ratio, shake at 300rpm for 5h and make Microspheres: collect the microspheres by centrifugation, after washing three times, transfer the microspheres to 100mL of 0.2% (0.1-1%) PVA solut...

Embodiment 2

[0041] Example 2. Preparation of PLG porous microsphere vaccine containing F1-antigen protein and its immune protection evaluation

[0042] 1. Preparation of PLG porous microsphere vaccine

[0043] 1. Preparation of PLG porous microspheres

[0044] Dissolve 500 mg of PLG in 2 mL of a mixture of acetone and dichloromethane (acetone-dichloromethane 1:1), then add this mixture to 200 μl of saturated sodium chloride solution, shake at 9000 rpm for 15 seconds to make colostrum; Cool the colostrum in an ice-water bath for 2 minutes, add 0.1% (0.1-2%) PVA solution containing 1% (1-10%) sodium chloride with a mass / volume ratio of 10 mL, shake at 8000 rpm for 15 seconds to make a compound Milk; then transfer the double milk to another 90mL of 0.1% (0.1-2%) PVA solution containing 1% (1-10%) sodium chloride with a mass / volume ratio of 1% (1-10%) sodium chloride, shake at 300rpm for 5h and make Microspheres: collect the microspheres by centrifugation, wash three times, transfer the mic...

Embodiment 3

[0058] Example 3, preparation of PLGA porous microsphere vaccine containing YscF-antigen protein and its immune protection evaluation

[0059] 1. Preparation of PLGA porous microsphere vaccine

[0060] 1. Preparation of PLGA porous microspheres

[0061] Dissolve 500mg of PLGA in 2mL of a mixture of acetone and dichloromethane (acetone-dichloromethane 1:1), then add this mixture to 200μl saturated sodium chloride solution, shake at 9000rpm for 15s to make colostrum; Cool the colostrum in an ice-water bath for 2 minutes, add 10 mL of 2% (0.1-2%) PVA solution containing 10% (1-10%) sodium chloride in mass / volume ratio, shake at 8000rpm for 15s to make a compound Milk; then transfer the double milk to another 90mL of 2% (0.1-2%) PVA solution containing 10% (1-10%) sodium chloride with a mass / volume ratio of 10% (1-10%) sodium chloride, and shake at 300rpm for 5h to prepare Microspheres: collect the microspheres by centrifugation, wash three times, transfer the microspheres to 10...

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Abstract

The invention discloses porous microspheres and the application thereof in preparing the vaccines of the porous microspheres. The porous microspheres are made through the following steps: biodegradable polymer material is dissolved in organic solvent to form mixed liquor, which is stirred and added into the saturated solution of sodium chloride to prepare protogala; and the protogala is added into PVA solution containing sodium chloride after being cooled by an ice water bath, thus preparing multiple emulsion; then the multiple emulsion is transferred into the PVA solution containing sodium chloride so as to prepare microspheres after oscillation; the microspheres are collected, washed and then transferred into the PVA solution to be shaken, finally, the microspheres are again collected by centrifugation, washed and freeze-dried. A freeze-dried preparation can be prepared by absorbing antigen protein of immunogenicity onto the porous microspheres to further obtain the vaccines of the porous microspheres. The invention provides a new way of preparing vaccines and has broad application prospect.

Description

technical field [0001] The invention relates to biodegradable porous microspheres and applications thereof, in particular to porous microspheres capable of absorbing antigenic proteins and their application in preparing porous microsphere vaccines. Background technique [0002] Plague is a severe infectious disease caused by Yersinia pestis. In recent years, the world's plague epidemic has been on the rise. In 2000, the World Health Organization listed plague as a re-emerging infectious disease. In addition, Yersinia pestis is a standard biological warfare agent and bioterrorist agent. The United States has listed Yersinia pestis as one of the six biological agents most likely to be used by terrorists. [0003] The inactivated plague vaccine only has a certain protective effect against bubonic plague, and the protection time is short, so this type of vaccine is not suitable for large-scale vaccination. Production of the USP inactivated vaccine used by the US military in th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/16A61K47/34A61K39/04A61P31/04
Inventor 王效义陆兵尚帅邱业峰王旺朱紫雯倪斌杨瑞馥
Owner MICROBE EPIDEMIC DISEASE INST OF PLA MILITARY MEDICAL ACAD OF SCI