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Antrodia camphoratea pimelie kelone compound for inhibiting B type hepatitis virus

A technology of hepatitis virus and compound, applied in the field of cyclohexenone compound, can solve the problems to be further experimentally studied and the like

Active Publication Date: 2012-05-30
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although it can be known from many current experiments that the extract of Antrodia Cinnamomea has the aforementioned effects, and its components have been analyzed one after another, but which active ingredients in the extract of Antrodia Cinnamomea can inhibit hepatitis B virus infection and treat hepatitis B Virus-related liver diseases still need further experimental research to clarify. Therefore, if the ingredients contained in the extract of Antrodia camphorata can be found to effectively inhibit the hepatitis B virus, it will be effective for acute hepatitis, chronic hepatitis, liver cirrhosis, and liver cancer. Treatment of hepatitis virus-associated liver disease would be of great help

Method used

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  • Antrodia camphoratea pimelie kelone compound for inhibiting B type hepatitis virus
  • Antrodia camphoratea pimelie kelone compound for inhibiting B type hepatitis virus
  • Antrodia camphoratea pimelie kelone compound for inhibiting B type hepatitis virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separate

[0028] Put about 100 grams of Antrodia antrodia mycelia, fruiting bodies or a mixture of the two into a conical flask, add an appropriate proportion of water and alcohols (for example, an aqueous alcohol solution of more than 70%), and store at 20-25 ° C. Stir and extract for at least 1 hour, then filter through filter paper and a 0.45 μm filter membrane to collect the extract.

[0029] The Antrodia camphorata extract collected above was analyzed by using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP18 chromatographic column (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B) were used as Mobile phase (mobile phase) (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio is 10%- 10%; 35-40 minutes, the B ratio is 10%-95%), eluting at a spee...

Embodiment 2

[0032] Toxicity Test of Cyclohexenone Compounds from Antrodia Antrodia on Liver Cancer Cells in Vitro

[0033] In order to further test the inhibitory effect of the Antrodia antrodia cyclohexenone compound on liver cancer tumor cells, this embodiment is carried out according to the National Cancer Institute (National Cancer Institute, NCI) anti-tumor drug screening mode, first take the implementation 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2 isolated in Example 1 - Cyclohexenone, added to the culture medium of HepG2 2.2.15 human liver tumor cells to test the viability of tumor cells. The test of cell viability can be analyzed by the well-known MTT assay method, and HepG2 2.2.15 is a human liver cancer tumor cell line, and the cell line can secrete hepatitis B virus with infectious ability.

[0034] MTT assay is a common analytical method used to analyze cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dim...

Embodiment 3

[0038] Effects of Cyclohexenone Compounds from Antrodia Antrodia on the Production of Hepatitis B Surface Antigen (HbsAg) and Hepatitis B Mantle Antigen (HbeAg) in Vitro

[0039] In order to test the influence of the cyclohexenone compound of Antrodia camphorata isolated and purified in Example 1 on the generation of hepatitis B surface antigen (HbsAg) and hepatitis B mantle antigen (HbeAg) produced by hepatitis B virus, the present embodiment first Human liver cancer cells HepG2 2.2.15 were cultured, and antrodia cyclohexenone compound was added to the culture medium of the cells, and then the type B in the culture medium was detected by semi-quantitative enzyme-linked immunoabsorbent assy (ELISA). Hepatitis Surface Antigen (HbsAg) and Hepatitis B Mantle Antigen (HbeAg) are produced. These two virus indicators are used to evaluate whether Antrodia antrodia cyclohexenone compound can effectively inhibit Hepatitis B virus. Among them, the HepG2 2.2.15 cell line uses the plasmid...

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Abstract

The invention relates to an antrodia camphorate cyclohexanedione compound for inhibiting B type hepatitis virus, in particular to a 4-hydroxy-2,3-dimethoxy-6-methy-5 (3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone separated from the antrodia camphorate extract, which can effectively inhibit the B type hepatitis virus. The invention has the advantages that the antrodia camphorate cyclohexanedione compound has cytotoxicity for the human hepatoma cell line HepG2 2.2.15 which can secrete B type hepatitis virus, and can reduce the formation amount of the B type hepatitis virus particle, and can effectively inhibit the B type hepatitis surface antigen (HbsAg) and the B type hepatitis mantle antigen (HbeAg), thus achieving the purpose of inhibiting the B type hepatitis virus.

Description

technical field [0001] The present invention relates to a compound for inhibiting viruses, in particular to a cyclohexenone compound isolated and purified from the extract of Antrodia camphorata and used for inhibiting hepatitis B virus. Background technique [0002] Hepatitis B virus (HBV), belonging to the Hepadnviridae family, is a small deoxyribonucleic acid viroid that can cause persistent noncytopathic liver infection and has a protein envelope (envelope) , and is currently the only DNA virus among human hepatitis viruses; the outer membrane of hepatitis B virus contains about 25% lipid and 75% glycoprotein, and the main component of glycoprotein is hepatitis B surface antigen (hepatitis B surface antigen; HbsAg) , while the core particle of hepatitis B virus contains hepatitis B envelope antigen (hepatitis B envelope antigen; HbeAg), among which, hepatitis B envelope antigen (HbeAg) is a secretory protein (secretory protein), which can pass through the When the place...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07C49/517A61K31/122A61P1/16A61P31/12A61P35/00
CPCA61K36/07C07C403/02C07C2101/16A61P1/16A61P31/12A61P35/00C07C2601/16
Inventor 刘胜勇温武哲郭茂田
Owner GOLDEN BIOTECH