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Extraction of astragalus polysaccharide and molecular modification method thereof

A technology of astragalus polysaccharides and modification methods, which is applied in the direction of pharmaceutical formulas, medical preparations containing active ingredients, plant/algae/fungus/moss components, etc., which can solve the problems of low biological activity and improve anti-virus and anti-virus Strong effect, antiviral and immune-enhancing activity

Inactive Publication Date: 2009-02-11
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0006] Aiming at the problem of low biological activity of traditional Chinese medicine polysaccharides, the present invention provides extraction and molecular modification methods of astragalus polysaccharides, so as to achieve the purpose of finding the most active astragalus polysaccharides and further improving the biological activity of astragalus polysaccharides

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  • Extraction of astragalus polysaccharide and molecular modification method thereof
  • Extraction of astragalus polysaccharide and molecular modification method thereof
  • Extraction of astragalus polysaccharide and molecular modification method thereof

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Embodiment Construction

[0021] 1. Extraction of Astragalus Polysaccharides

[0022] Take 4kg of Inner Mongolia Astragalus and add water in a ratio of 1:8, boil it with a fire to boil, then simmer for 1.0h, filter out the medicinal residue, add the medicinal residue in a ratio of 1:6 and then decoct it in the same way 2 times, combine 3 times of filtrate, heat and concentrate to 4kg (1g crude drug / mL).

[0023] (1) One-step alcohol precipitation method: Take 1kg of the medicinal solution and slowly add ethanol once to make the final concentration reach 70%, stir, stand for precipitation for 24h, and filter with suction. Take the precipitate and add 5 times the volume of distilled water to dissolve it, let it stand for 5h, and centrifuge at 3000r / min for 10min to remove the precipitate. The supernatant was repeatedly precipitated once, and the precipitate was dried at 60°C for 3 days to obtain crude total astragalus polysaccharide (APSt).

[0024] (2) Step-by-step alcohol precipitation method: Take 3kg of ...

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Abstract

The invention discloses a method for extracting astragalus polysaccharide and modifying molecular, pertaining to the technical field of preparation and structure reform of Chinese medicine polysaccharides. A chlorosulfonic acid-pyridine method is used for modifying sulfating molecular, amount of products and substituting degree of sulfate radical are used as indicators, and reaction temperature, reagent mixture ratio and reaction time serve as factors. An orthogonal test is used for determining a preferential best condition of astragalus polysaccharides chlorosulfonic acid-pyridine method that the reaction temperature is 95 DEG C, the mixture ratio of chlorosulfonic acid and pyridine is 1:6 and the reaction time is 1h. Astragalus decoction is slowly added into ethanol for the first time till the final concentration is up to 50 percent, and sediment is removed; supernatant fluid is added into the ethanol till the final concentration is up 60 percent and sediment is taken to obtain crude polysaccharide. After purification, the astragalus polysaccharide is modified by an optimized condition. Through a series of comparisons between antivirus and strengthening immunocompetence, the astragalus polysaccharide has the strongest antivirus and strengthening immunocompetence by purification and sulfating modification.

Description

1. Technical Field [0001] The invention is a method for extracting and molecular modification of Astragalus polydsaccharide (APS), and belongs to the research field of improving the structure modification of traditional Chinese medicine polysaccharide. 2. Background technology [0002] Polysaccharides are another type of important biological macromolecules in organisms besides proteins and nucleic acids. They can resist infection, enhance immunity, prevent tumors and viral hepatitis, rheumatoid disease, AIDS and other immune damage or immunodeficiency and anti-oxidation. Aspects of function and biological activity. With the rapid development of chemistry and biology and the improvement of separation technology, the biological function of polysaccharides, especially as a living substance, participates in all time and space functions of life, breaking through the traditional concept of polysaccharides as supporting tissues and energy sources. With a wider range of biological effect...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/00A61K36/481A61P31/12A61P37/04
Inventor 胡元亮黄小燕王德云张宝康刘家国
Owner NANJING AGRICULTURAL UNIVERSITY
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