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2-keto-d-gluconic acid continuous fermentation process

A technology of gluconic acid and glucose, applied in the directions of fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low fermentation yield, rising production cost, loss of production capacity of fermentation equipment, etc.

Inactive Publication Date: 2009-02-11
江西省德兴市百勤异VC钠有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After each batch of fermentation is completed, the fermenter needs to be cleaned and re-inoculated before the next batch of fermentation can be carried out, resulting in an increase in production costs and a loss of production capacity of fermentation equipment
In terms of fermentation conversion rate, although the existing 2-keto-D-gluconic acid fermentation technology has reached a certain level; but in terms of fermentation yield and product quality, the existing 2-keto-D-gluconic acid fermentation technology There are still some deficiencies in the technology, which are manifested in the low fermentation yield, generally only reaching about 4.0g / L / h, and the product quality is not stable enough

Method used

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  • 2-keto-d-gluconic acid continuous fermentation process

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] In this embodiment, a 5L self-controlled fermenter was used for seed cultivation. Put the bacterial suspension of Pseudomonas fluorescens K1005 into 3.5L containing 2.0% glucose, 1.0% corn steep liquor, 0.2% urea, 0.2% KH 2 PO 4 , 0.05% MgSO 4 ·7H 2 O, 0.25% light CaCO 3 In the seed medium (pH 7.0 before sterilization), the stirring speed was controlled to 400 rpm, the tank temperature was 33° C., the tank pressure was 0.030 MPa, and the ventilation rate was 0.5 v / v / m for 20 h.

[0022] The continuous fermentation adopts a 50L self-controlled fermenter, which has a stirring system and a control and monitoring system for temperature, pH, and dissolved oxygen (DO). Its working volume is 35L, the temperature is controlled at 33±1°C, the stirring speed and ventilation rate are set at 400rpm and 3.5L / min, respectively. During the continuous fermentation process, use a peristaltic pump to control the feeding rate, and use another peristaltic pump to continuously discharg...

Embodiment 2

[0025] Seed culture and fermentation conditions are controlled with embodiment 1.

[0026] The composition of the fermentation medium was: 18.0% starch hydrolyzed sugar, 1.7% corn steep liquor and 5.0% light CaCO 3 (pH6.7 before sterilization). Put the cultivated seed liquid into 35L fermentation medium and cultivate for 30h, then switch to continuous fermentation mode, set the dilution rate to 0.055h -1 , Continuous feeding of fermentation medium at a feed rate of 1.925 L / h. Continuous fermentation was carried out for 120 hours in total, producing 45916.9g of 2-keto-D-gluconic acid, the concentration of 2-keto-D-gluconic acid in the fermentation broth was 173.6g / L, and the average residual glucose in the fermentation broth was 0.011% . Calculated according to the above results, the average fermentation conversion rate (mol / mol) is 88.98%, and the average fermentation yield is about 6.12g / L / h.

Embodiment 3

[0028] Seed culture and fermentation conditions are controlled with embodiment 1.

[0029] The composition of the fermentation medium is: 18.0% starch hydrolyzed sugar and 1.5% corn steep liquor (pH4.5 before sterilization, adjusted to 6.2 with sterile sodium hydroxide solution after sterilization). Put the cultured seed liquid into 35L fermentation medium for cultivation, and use sodium hydroxide solution to control the pH of the fermentation liquid to about 5.3, and switch to continuous fermentation mode after 30h, and set the dilution rate to 0.055h -1 , Continuous feeding of fermentation medium at a feed rate of 1.925 L / h. Continuous fermentation was carried out for 144 hours in total, producing 54072.8g of 2-keto-D-gluconic acid, the concentration of 2-keto-D-gluconic acid in the fermentation broth was 173.3g / L, and the average residual glucose in the fermentation broth was 0.009% . Calculated according to the above results, the average fermentation conversion rate (mol...

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Abstract

A method for fermenting and producing 2-ketone group-D-gluconic acid and salts thereof from glucose or starch hydrolysate sugar by continuous fermentation with high yield, comprises the steps that bacterium is transformed into the 2-ketone group-D-gluconic acid by using the glucose or the starch hydrolysate sugar is cultivated in a fermentation vessel; culture medium containing glucose is continuously supplied into the fermentation vessel; by being corresponding to the rate of dilution of zymotic fluid, zymotic fluid is continuously discharged out from the fermentation vessel; the 2-ketone group-D-gluconic acid or the salts thereof is recycled from the zymotic fluid which is continuously discharged out.

Description

technical field [0001] The invention relates to a continuous fermentation production process of food additive D-erythorbic acid and its salts synthetic precursor 2-keto-D-gluconic acid. Background technique [0002] 2-Keto-D-gluconic acid is the precursor for the synthesis of D-erythorbic acid and its salts (such as D-erythorbic acid sodium), D-erythorbic acid and its salts can be used as food antioxidants and preservatives for meat products Toner. [0003] 2-Keto-D-gluconic acid can be converted into D-erythorbic acid and its salts (such as D-sodium erythorbate) according to known indirect fermentation techniques (such as US3,381,027). The production methods of 2-keto-D-gluconic acid mainly include enzymatic method (such as US4,351,902), chemical synthesis method (such as US6,018,034) and fermentation method (such as US3,255,093). Due to production cost reasons, bacterial fermentation methods are currently used in industrial production. [0004] The known 2-keto-D-glucon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/58C12R1/39
Inventor 余泗莲孙文敬周强杨晓军余彬李中兵魏转刘敬泽黄达明
Owner 江西省德兴市百勤异VC钠有限公司
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