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Process for preparing bacillus subtilis alkali proteinase with microorganism zymotechnics

A technology of Bacillus subtilis and microbial fermentation, which is applied in the production process of alkaline protease, can solve problems such as high cost, limited output, and limited enzyme application range, and achieve low production cost, short production cycle, and general equipment requirements. Effect

Inactive Publication Date: 2011-03-30
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, animal pancreas is mostly used for extraction and production at home and abroad. The cost is high and the output is limited, which limits the scope of application of this enzyme.

Method used

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  • Process for preparing bacillus subtilis alkali proteinase with microorganism zymotechnics
  • Process for preparing bacillus subtilis alkali proteinase with microorganism zymotechnics
  • Process for preparing bacillus subtilis alkali proteinase with microorganism zymotechnics

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0057] (1) Strain culture:

[0058] ①Seed medium:

[0059] LB liquid medium: peptone 10g, yeast extract 5g, NaCl 10g, water 1L, pH7.0, autoclaved at 121°C for 30min.

[0060] ② Expansion of training:

[0061] Use an inoculation loop to pick up a ring of BQ-An strain sludge stored on the slope, and inoculate it in a 250mL Erlenmeyer flask containing 100mL of seed medium. Cultivate for 36-38 hours under the condition of 38°C constant temperature shaker and 160r / min.

[0062] (2) Fermentation medium preparation:

[0063] a: The optimal formula (w / w) of the fermentation medium is: glucose 1.2% + sucrose 0.6% + starch 1.2%, bean cake powder 3%, yeast powder 0.3%, K 2 HPO 4 ·3H 2 O0.2%; the rest supplemented with distilled water. b: adjust the pH of the fermentation medium prepared according to the above formula to 7.8, pack it into a triangular flask, sterilize it at 0.105Mpa steam pressure for 30 minutes, cool it naturally to 45°C, and inoculate Bacillus subtilis strains un...

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Abstract

The invention relates to a production process for producing Bacillus subtilis alkali protease with the microbiological fermentation method, which adopts microbiological fermentation to produce alkali protease, the period is short, the nutrition requirements are low, the extraction process is simple, the cost is reduced, and at the same time, the recovery rate of the protease is improved. The production process comprises the following steps: (1) strain culture: seed medium is adopted to carry out the amplification culture of Bacillus subtilis strains; (2) preparation of fermentation medium; (3) fermentation: the Bacillus subtilis strains are inoculated to a shaking flask to ferment and obtain fermentation liquid; (4) crude extraction: after being pretreated, the fermentation liquid is centrifuged, decolored, filtered, decompressed and dried to obtain crude alkali protease; and (5) refining: after being purified and dissolving, the crude alkali protease is undergone the salting out, dialysis, ultrafiltration concentration and gel permeation chromatography, and then is dried and concentrated to obtain the finished product.

Description

1. Technical field: [0001] The invention relates to a production process of alkaline protease, in particular to a production process for producing bacillus subtilis alkaline protease by microbial fermentation. 2. Background technology: [0002] With the increase in the types and output of chemical pesticide products, the scope of their application is also expanding. Pesticide residues have caused serious threats to human beings and the ecological environment. Biopesticides are highly effective, safe, non-toxic, non-residual, and non-environmental. It has become the most active research field in agricultural development. Over the past ten years, due to the attention of various countries, the research and production of biological pesticides have made great progress. At present, chemical pesticides are decreasing at a rate of 2% per year, while biological pesticides are increasing at a rate of 5%. In particular, my country, as a large agricultural country, faced fierce competi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12R1/125C12N9/56
Inventor 安德荣刘双发
Owner NORTHWEST A & F UNIV
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