SG fusion protein

A technology of fusion protein and protein, applied in the field of fusion protein, can solve problems such as poor curative effect, leukopenia, and unsatisfactory effect

Inactive Publication Date: 2009-02-25
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

It has been clinically used in the hematopoietic recovery of patients with radiotherapy and chemotherapy, but the effect is not very sati

Method used

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Embodiment 1

[0036] Embodiment 1: Preparation of SG fusion protein

[0037] 1. Preparation of PPIC9K-SDF1-rhGM-CSF1 clone:

[0038] 1) The SDF1-rhGM-CSF1 gene was synthesized by Invitrogen Company and loaded in the pMD18-T (purchased from TAKARA Company) vector. Digest pMD18-T-SDF1-rhGM-CSF1 with EcoRI and Xho I (product of TAKARA company), and recover small fragments by tapping the rubber to obtain the SDF1-rhGM-CSF1 gene fragment with EcoRI and Xho I multiple cloning sites at both ends .

[0039] 2) The pPCI9K plasmid (purchased from Invitrogen) was also subjected to double enzyme digestion with EcoRI and Xho I and then subjected to agarose gel electrophoresis. The results showed that the band size was correct. After cutting the gel, it was recovered with the Axygen Gel Recovery Kit.

[0040] The pMD18-T-SDF1-rhGM-CSF1 and pPCI9K plasmid digestion reaction system is as follows:

[0041]

[0042] 3) Ligate the digested fragment SDF1-rhGM-CSF1 and the carrier PPIC9K with T4DNALigase (p...

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Abstract

The invention relates the technical field of medical biology; currently, the medicines which is often used for hematopoiesis and restoring injured tissues clinically are GM-CSF, G-CSF and GM-CSF that mainly take effect on colony forming unit-granulocyte-monocyte to promote proliferation and differentiation, so as to have action on hemopoietic stem cell, medullary system multipotent progenitor cell as well as erythroid and macronucleus system hematopoiesis. But the effect of promoting hematopoiesis and proliferation of the medicines is provisional, the maintaining time is short, and the effects disappear when the medicines are stopped being taken. The invention provides a fusion protein which is used for preventing and treating hematopoiesis damage caused by radiation treatment and chemo-treatment and has the function of enhancing immunity. The gene of the SG fusion protein of the invention has the nucleotide sequence which is shown as SEQ ID NO: 4 and has the whole length of 850bp. The experiments of biology in vitro shows that the SG fusion protein of the invention can promote bone marrow colony cells to form, can effectively lead immature dendritic cells to be chemotactic, and has stronger function compared with GM-CSF.

Description

Technical field: [0001] The invention relates to the technical field of medical biology, and is a fusion protein that can be used to prevent and treat hematopoietic damage caused by radiotherapy and chemotherapy and has the function of enhancing immunity. Background technique: [0002] Radiotherapy and chemotherapy are effective means to treat a variety of malignant tumors. The hematopoietic system of tumor patients is most likely to be damaged during radiotherapy and chemotherapy, and immune function declines at the same time. At present, the application of concurrent radiotherapy and chemotherapy has improved the survival rate of cancer patients, but the hematopoietic and immune system damage of the patients is aggravated, which increases the incidence of complications such as infection and bleeding, and affects the curative effect. Therefore, it is crucial to restore the hematopoietic function as soon as possible important. At present, GM-CSF and G-CSF are commonly used ...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/62A61K38/19A61P7/00A61P37/04
Inventor 居小萍张晓青徐斌刘永明陈樱
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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