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Tissue engineering reactor having tension-compression and cultivation cavity rotation functions

A tissue engineering and rotational function technology, which is applied in specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, tissue cell/virus culture device, etc. The effect of good controllability, great promotion and application prospects, and good mass transfer performance

Active Publication Date: 2009-02-25
BEIHANG UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] To sum up, the existing tissue engineering reactors have major defects: first, they cannot provide the tissue with mechanical loads such as periodic axial tension and compression, compression, radial stretch, and twisting at the same time; Most of them adopt the method of rotation without wall surface, which needs to be improved in terms of uniform cell, high-density seeding and mass transfer

Method used

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  • Tissue engineering reactor having tension-compression and cultivation cavity rotation functions
  • Tissue engineering reactor having tension-compression and cultivation cavity rotation functions
  • Tissue engineering reactor having tension-compression and cultivation cavity rotation functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] Example 1 External rotation tension and pressure perfusion

[0063] 1. According to figure 1 The tissue culture cavity perfusion circuit is connected as shown, wherein the liquid driving device is a peristaltic pump (Cole-Parmer Company, Masterflex series);

[0064] 2. Sterilize the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0065] 3. Install the bone tissue culture to be cultured on the jig in the tissue culture chamber in a sterile state, wherein the bone tissue culture has a diameter of 4 mm and a length of 5 cm, and the scaffold material is hydroxyapatite;

[0066] 4. According to figure 1 The tissue culture chamber rotation device is connected as shown, wherein the tissue culture chamber rotation motor is a Haydn 57000 series stepping motor;

[0067] 5. According to figure 1 The connecting bone tissue pulling and pressing device is shown, wherein the bone tissue pulling and pressing motor is a Haydn 57000 series linear stepper mot...

Embodiment 2

[0074] Embodiment 2 External rotation tension and pressure perfusion belt control system

[0075] 1. According to figure 2 The tissue culture cavity perfusion circuit is connected as shown, wherein the liquid driving device is a peristaltic pump (Cole-Parmer Company, Masterflex series);

[0076] 2. Sterilize the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0077] 3. Install the bone tissue culture to be cultured on the jig in the tissue culture chamber in a sterile state, wherein the bone tissue culture has a diameter of 4 mm and a length of 5 cm, and the scaffold material is hydroxyapatite;

[0078] 4. According to figure 2 The tissue culture chamber rotation device is connected as shown, wherein the tissue culture chamber rotation motor is a Haydn 57000 series stepping motor;

[0079] 5. According to figure 2 The pressure sensor connected to the inlet and outlet of the tissue culture chamber as shown, and the signal detection device;

[0...

Embodiment 3

[0088] Example 3 Control system of external rotation tension and pressure perfusion internal rotation belt

[0089] 1. According to figure 2 The tissue culture cavity perfusion circuit is connected as shown, wherein the liquid driving device is a peristaltic pump (Cole-Parmer Company, Masterflex series);

[0090] 2. Sterilize the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0091] 3. Install the bone tissue culture to be cultured on the jig in the tissue culture chamber in a sterile state, wherein the bone tissue culture has a diameter of 4 mm and a length of 5 cm, and the scaffold material is hydroxyapatite;

[0092] 4. According to image 3 The tissue culture rotating device and the tissue culture chamber rotating device are connected as shown, wherein the tissue culture rotating motor adopts Haydn 57000 series stepper motor; the tissue culture chamber rotating motor adopts Haydn 57000 series stepping motor;

[0093] 5. According to figure ...

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Abstract

The invention provides a tissue engineering reactor with the functions of tension, compression, and rotation of a culture cavity. The tissue engineering reactor comprises a tissue culture cavity (103), a culture solution perfusion circuit which penetrates through the tissue culture cavity (103), a first holding device (108) and a second holding device (108) which are arranged inside the tissue culture cavity (103) and used for arranging tissue culture to be cultured between the first holding device and the second holding device, a tissue culture tension and compression driving device which iscoupled with the first holding device (108) and used for driving the first holding device (108) to do reciprocating motion so as to carry out tension and compression on the tissue culture, and a culture cavity rotation driving device which is coupled with the tissue culture cavity (103) and used for driving the tissue culture cavity (103) to rotate. The tissue engineering reactor can load mechanical loading on the tissue culture such as periodical axial tension and compression, and the like, in the course of culture, and a culture chamber of the reactor is designed in a wall rotating-direct perfusion mode, which ensures good mass transfer performance.

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more specifically relates to a tissue engineering reactor with the functions of tension and compression and rotation of a culture chamber. Background technique [0002] Tissue engineering refers to the combination of engineering and life science principles and methods to prepare tissue and organ substitutes to restore, maintain or improve the functions of human tissues and organs. It is a rapidly developing and far-reaching biomedical engineering. The field of application is a new branch of biomedical engineering. The emergence and development of tissue engineering technology has created unprecedented opportunities for the treatment of congenital and acquired diseases. [0003] Tissue engineering bioreactor is an important part of tissue engineering model system. It can simulate some biochemical and physical signals that regulate tissue development and maintenance in vivo, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34
CPCC12M35/04C12M21/08
Inventor 樊瑜波黄学进邹远文李晋川贡向辉
Owner BEIHANG UNIV