Mycobacterium tuberculosis rapid drug sensitive detection reagent kit and detection method thereof

A technology of Mycobacterium tuberculosis and detection kit, applied in measurement devices, analysis materials, material analysis by optical means, etc., can solve the problem of high mortality rate of tuberculosis, it takes 2-3 months to get results, and affects the timely and correctness of patients. Medication and other issues, to achieve the effect of increasing the positive detection rate and getting the results earlier

Inactive Publication Date: 2009-03-04
AUTOBIO DIAGNOSTICS CO LTD
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Problems solved by technology

Due to the slow growth of Mycobacterium tuberculosis, it generally takes about 20-30 days, and the drug sensitivity test also takes 20-30 days, and the whole drug sensitivity test takes 2-3 months to get the results, which seriously affects the timel...

Method used

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Embodiment 1

[0019] The kit used in the present invention includes specimen processing reagents, separation and purification reagents, drug sensitivity culture reagents, and cell activity detection reagents. Wherein the sample processing reagent is 4% sodium hydroxide and N-acetyl-L-cysteine; the separation and purification reagent is the nano-immunomagnetic beads coated with the specific membrane antibody of Mycobacterium tuberculosis; the The drug susceptibility culture reagent used is 7H9 medium, which contains antibiotics streptomycin, rifampicin, isoniazid, ethambutol, and levofloxacin for anti-mycobacterium tuberculosis. The antibiotics are divided into low concentration and high concentration. There is a blank control. The cell viability detection reagent is alamar blue indicator. The detection process is as follows: the specific membrane antibody of Mycobacterium tuberculosis is coated on the nano-immunomagnetic beads for standby. Take a sputum sample from a patient with positive...

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Abstract

The invention relates to a rapid drug sensitive detection reagent kit for Mycobacterium tuberculosis and a detection method thereof. The rapid drug sensitive detection reagent kit for Mycobacterium tuberculosis contains specimen treatment reagents, purification reagents, drug sensitive culture reagents and cell activity detection reagents; wherein, the cell activity detection reagents are alamar blue indicators. With the reagent kit and a result analysis method of the invention, the positive rate is greatly improved in comparison with the traditional method, the time of obtaining results is greatly advanced, and the whole test can obtain the results within three days.

Description

(1) Technical field [0001] The invention belongs to the field of mycobacterium drug sensitivity detection, and in particular relates to a rapid drug sensitivity detection kit of mycobacterium tuberculosis and a detection method thereof. (2) Background technology [0002] As an ancient infectious disease, tuberculosis is on the rise globally. According to WTO estimates, about 2 billion people in the world are infected with Mycobacterium tuberculosis, with 8 million new cases and 3 million deaths every year. In my country, according to the fourth national tuberculosis survey in 2000, there were about 550 million tuberculosis cases in the country, nearly half of the country's population. The current situation of tuberculosis in my country is severe. It is an urgent task to establish advanced diagnostic techniques and treatment measures as soon as possible. It is not only related to the physical and mental health of tens of millions of people, but also an important issue rela...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N21/00
Inventor 付光宇娄斌苗兰芳胡英会陆影楠吴学炜
Owner AUTOBIO DIAGNOSTICS CO LTD
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