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Tissue engineering reactor with torsion and tension-compression functions

A technology of tissue engineering and reactor, which is applied in the direction of specific-purpose bioreactor/fermenter, bioreactor/fermenter combination, tissue cell/virus culture device, etc. The effect of great potential social and economic benefits, great promotion and application prospects, and good mass transfer performance

Active Publication Date: 2009-03-11
BEIHANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0013] To sum up, the existing tissue engineering reactors have major defects: first, they cannot provide the tissue with mechanical loads such as periodic axial tension, compression, radial stretch, and twisting that approximate the physiological state at the same time; Most of them adopt the method of rotation without wall surface, which needs to be improved in terms of uniform cell, high-density seeding and mass transfer

Method used

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  • Tissue engineering reactor with torsion and tension-compression functions
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  • Tissue engineering reactor with torsion and tension-compression functions

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0067] Example 1 Twisted tension and pressure perfusion

[0068] 1. According to figure 1 The tissue culture cavity perfusion circuit is connected as shown, wherein the liquid driving device is a peristaltic pump (Cole-Parmer Company, Masterflex series);

[0069] 2. Sterilize the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0070] 3. Install the bone tissue culture to be cultured on the jig in the tissue culture chamber in a sterile state, wherein the bone tissue culture has a diameter of 4 mm and a length of 5 cm, and the scaffold material is hydroxyapatite;

[0071] 4. According to figure 1 The tissue culture torsion device is connected as shown, wherein the tissue culture torsion motor is a Haydn 57000 series stepper motor;

[0072] 5. According to figure 1 The connecting bone tissue pulling and pressing device is shown, wherein the bone tissue pulling and pressing motor is a Haydn 57000 series linear stepper motor;

[0073] 6. Prepare the ...

Embodiment 2

[0079] Example 2 Twisted tension and pressure perfusion belt control system

[0080] 1. According to figure 2 The tissue culture cavity perfusion circuit is connected as shown, wherein the liquid driving device is a peristaltic pump (Cole-Parmer Company, Masterflex series);

[0081] 2. Sterilize the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0082] 3. Install the bone tissue culture to be cultured on the jig in the tissue culture chamber in a sterile state, wherein the bone tissue culture has a diameter of 4 mm and a length of 5 cm, and the scaffold material is hydroxyapatite;

[0083] 4. According to figure 2 The tissue culture torsion device is connected as shown, wherein the tissue culture torsion motor is a Haydn 57000 series stepper motor;

[0084] 5. According to figure 2 The pressure sensor connected to the inlet and outlet of the tissue culture chamber as shown, and the signal detection device;

[0085] 6. According to figure 2 ...

Embodiment 3

[0093] Example 3 Twisted tension and pressure perfusion belt control system

[0094] 1. According to Figure 4The tissue culture cavity perfusion circuit is connected as shown, wherein the liquid driving device is a peristaltic pump (Cole-Parmer Company, Masterflex series);

[0095] 2. Sterilize the reactor, the disinfection conditions are: 130°C, 3 atmospheres, 1 hour;

[0096] 3. Install the bone tissue culture to be cultured on the jig in the tissue culture chamber in a sterile state, wherein the bone tissue culture has a diameter of 4 mm and a length of 5 cm, and the scaffold material is hydroxyapatite;

[0097] 4. According to Figure 4 The connection tissue culture tension, compression and twisting device is shown, wherein the tissue culture tension, compression and twisting motor is a dual-motion motor;

[0098] 5. According to Figure 4 The pressure sensor and signal detection device connected to the inlet and outlet of the tissue culture chamber are shown;

[009...

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Abstract

The invention provides a tissue engineering reactor with functions of torsioning and drawing, which comprises a tissue culture cavity, a culture fluid perfusion loop, a first maintaining device and a second maintaining device, a drawing driver of tissue culture and a torsioning driver of the tissue culture, wherein the culture fluid perfusion loop passes through the tissue culture cavity; the first maintaining device and the second maintaining device are arranged in the tissue culture cavity for arranging the tissue culture between the first maintaining device and the second maintaining device; the drawing driver of the tissue culture is coupled with at least one of the first maintaining device and the second maintaining device for driving the maintaining device to make reciprocating motion so as to draw the tissue culture; and the torsioning driver of the tissue culture is coupled with at least one of the first maintaining device and the second maintaining device for driving the maintaining device to rotate, so that the tissue culture arranged between the first maintaining device and the second maintaining device can be twisted. The reactor can load periodical axial drawing, twisting and other mechanical loading on the culture while culturing, and has excellent mass transfer performance and regulating and controlling capability.

Description

technical field [0001] The invention relates to the fields of cell culture and tissue engineering, and more specifically relates to a tissue engineering reactor with the functions of torsion and compression for tissue culture. Background technique [0002] Tissue engineering refers to the combination of engineering and life science principles and methods to prepare tissue and organ substitutes to restore, maintain or improve the functions of human tissues and organs. It is a rapidly developing and far-reaching biomedical engineering. The field of application is a new branch of biomedical engineering. The emergence and development of tissue engineering technology has created unprecedented opportunities for the treatment of congenital and acquired diseases. [0003] Tissue engineering bioreactor is an important part of tissue engineering model system. It can simulate some biochemical and physical signals that regulate tissue development and maintenance in vivo, and provide a v...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M3/00C12M1/34
CPCC12M21/08C12M35/04
Inventor 樊瑜波贡向辉
Owner BEIHANG UNIV
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