Application of duel functional composite bacteria agent capable of decomposition of stalk and low-temperature production of biogas
A compound inoculant and biogas production technology, applied in the directions of bacteria, microorganism-based methods, biochemical equipment and methods, etc., can solve the problems of difficult fermentation of straw biogas, low-temperature gas production, etc., and achieves low cost, easy popularization, and usage simple effect
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Embodiment 1
[0032] Example 1: Straw decomposition and low-temperature biogas-producing dual-functional composite bacterial agent, straw decomposition and low-temperature biogas-producing dual-functional composite bacterial agent is composed of Pseudomonas fluorescens, Bacillus cereus, and Bacillus subtilis The fermented liquid of four kinds of bacteria of Bacillus subtilis and Bacillus circulans consists of the following parts by weight:
[0033] 6-15 parts of Pseudomonas fluorescens liquid, 4-8 parts of Bacillus cereus liquid, 6-10 parts of Bacillus subtilis liquid, and 5-8 parts of Bacillus circulus liquid.
[0034] The above four kinds of bacteria are all purchased from the strain preservation center, and are fermented and prepared by conventional methods, and the number of effective viable bacteria in the fermentation broth is respectively above 1 billion / ml.
Embodiment 2
[0036] Example 2: A method of using a dual-functional bacterial agent for straw decomposition and low-temperature biogas production in an 8m3 biogas digester with straw as the main raw material, the steps are as follows:
[0037] 1. Select 400kg of corn stalks free from pesticides, disinfectants and other toxic substances as fermentation raw materials.
[0038] 2. Shred the straw raw materials with a hay cutter, and the length is 1-3cm.
[0039] 3. Add 400kg of water to moisten the straw raw material, turn over and mix well while adding water, and then place the straw for one day to make the straw fully absorb water.
[0040] 4. Mix 5 kg of ammonium bicarbonate with a small amount of water and sprinkle it into the wet straw in layers to make the moisture content of the straw reach 70%.
[0041] 5. Pile the straw into a length of 1-1.2m, a width of 1-1.2m, and a height of 1-1.2m, and cover it with plastic cloth for retting. Straw raw materials are turned into the pile for retti...
Embodiment 3
[0045] Example 3: A dual-functional microbial agent for straw decomposition and low-temperature biogas production was used in a 10m2 plant with straw as the main raw material 3 The method of use in the biogas digester, the steps are as follows:
[0046] 1. Select 500kg of corn stalks free from pesticides, disinfectants and other toxic substances as fermentation raw materials.
[0047] 2. Shred the straw raw materials with a hay cutter, and the length is 1-3cm.
[0048] 3. Add 500kg of water to moisten the straw raw material, turn over and mix well while adding water, and then place the straw for one day to make the straw fully absorb water.
[0049] 4. Mix 6 kg of ammonium bicarbonate with a small amount of water and sprinkle it into the wet straw in layers to make the moisture content of the straw reach 70%.
[0050] 5. Pile the straw into a length of 1-1.2m, a width of 1-1.2m, and a height of 1-1.2m, and cover it with plastic cloth for retting. Straw raw materials are tur...
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