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Method for increasing abundance and stability of antibacterial peptide expressed inside plant

An antibacterial peptide and stability technology is applied in the field of enhancing the abundance and stability of the expression of antibacterial peptides in plants, and can solve the problems of low expression abundance of heterologous antibacterial peptides and degradation of plant endogenous proteases.

Inactive Publication Date: 2009-04-01
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Problems solved by technology

[0005] The invention provides a method for enhancing the abundance and stability of antimicrobial peptides expressed in plants, which solves the problem that heterologous antibacterial peptides are expressed in plants with low abundance and are easily degraded by endogenous plant proteases

Method used

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  • Method for increasing abundance and stability of antibacterial peptide expressed inside plant
  • Method for increasing abundance and stability of antibacterial peptide expressed inside plant
  • Method for increasing abundance and stability of antibacterial peptide expressed inside plant

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Embodiment Construction

[0010] Taking the antimicrobial peptide Thanatin as an example, a multi-copy antimicrobial peptide fusion expression vector was constructed.

[0011] Key points of the design: the cleavage site of the antibacterial peptide of Impatiens is used as the spacer sequence, the amino acid sequence and cleavage method are S↓NAADEVATPE↓DVEPG, the signal peptide of soybean chitinase is used as the guide sequence, and the sequence characteristics are MASERQALMLILL TTFF FTIKPSQA , the multi-copy antimicrobial peptide fusion expression product is introduced into the intercellular space and accumulates in high abundance around the cell wall, and then cleaved into a single active antimicrobial peptide unit. The fusion expression study of the soybean chitinase signal peptide and GFP protein shows that the signal peptide can specifically introduce the target protein into the cell space and cell wall.

[0012] Attached figure 1 Design antimicrobial peptide fusion expression vector structure. ...

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Abstract

The invention relates to a method for strengthening the expression abundance and stability of antibacterial peptide in plants, which belongs to the field of biotechnology and by the design of fusion expression technology in series of multicopy antibacterial peptide which can automatically cut in plants, the antibacterial peptide expressed by fusion is led to be automatically cut into single antibacterial peptide units with functional activity in plants so as to increase the expression abundance and stability of the antibacterial peptide.

Description

technical field [0001] The invention relates to a technology for expressing antimicrobial peptides in series and fusion in plants to improve the abundance and stability of antimicrobial peptide expression, which is used to enhance the disease resistance of crops. Background technique [0002] Antimicrobial peptides are a class of peptides produced in the defense system of organisms that have high-efficiency killing activity against exogenous bacteria, especially some animal-derived antimicrobial peptides, such as cecropins, melittins, and bombesin (Magainins), etc., have certain killing activity on various pathogenic bacteria such as bacteria, fungi, viruses, spirochetes, and even cancer cells, and long-term use will not cause drug resistance of pathogenic bacteria, and there is no residual pollution problem. It is an antibiotic and Ideal alternative to chemical fungicides. [0003] Due to the advantages of high efficiency, environmental protection, and no resistance to pat...

Claims

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Application Information

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IPC IPC(8): C12N15/09C12N15/62C12N15/82
Inventor 刘仁虎陈锦清汪小福陈笑芸王伏林刘智宏郎春秀
Owner ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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