Method for preparing mannanase by using glycerol as carbon source and fedbatch of substrate for induction

Abstract

The invention relates to a preparation method for mannanase by induction of a substrate batch fed into a carbon source of glycerol, in particular to a fermentation production process for genetically engineered strain of the mannanase with Pichia stipitis as a carrier. The invention is characterized in that a seed culture solution is used for culture in a first-stage seed culture fermentor; a basic culture solution is used for culture in a second-stage seed fermentor; part of the basic culture solution is poured into a production fermentor for culture at initial time, and the glycerol, methanol and mannan substrate are respectively fed into a fermentation solution to finish the fermentation at a certain proportion and speed in the fermentation process. The yield of the mannanase can be obviously increased by 15 to 24 percent.

Description

technical field

[0001] The invention relates to a fermentation technology of microbial products, a method for inducing and preparing mannanase by using glycerol as a carbon source and feeding substrates. Background technique

[0002] The traditional method of producing mannanase uses glucose as a carbon source. Glucose, as a carbon source, produces caramelization during the high-temperature sterilization process, making the fermentation medium solution brown or soy sauce-colored. Its disadvantages are: first, the caramelization reaction produces harmful substances, which is not conducive to the growth of yeast; second, the post-processing of the dark fermented liquid requires decolorization, which increases the production process and costs. Third, the induction is performed without adding the substrate mannan, and the yield is low. Contents of the invention

[0003] The purpose of the present invention is to provide a method for inducing and preparing mannanase by using ...

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