Salt resistant endo cellulose and coding gene thereof

Abstract

The invention belongs to the technical field of gene engineering, in particular to a new salt resistant endo cellulase and a coding gene thereof. The invention discloses a sequence and a coding area of the gene; the sequence of the gene is shown as SEQ ID NO: 1; the full length of the sequence is 1053bp; and 350 amino acids are coded. Escherichia coli DH5 Alpha / S664 / 6p / cel8h containing a gene plasmid is preserved in China Center for Type Culture Collection (CCTCC); and the serial number of preservation is CCTCC NO:M208233. Biology validates and shows that the endo cellulase has strong tolerance to high saline salinity. The endo cellulase can be widely applied in high saline catalysis, cellulose fermentation and other industrial fields.

Description

technical field The invention relates to the technical field of genetic engineering, in particular to a new salt-tolerant endo-cellulase and the cloning of its coding gene. technical background In today's world, energy problems are becoming more and more serious. Searching for sustainable bio-energy has become the focus of competition among countries. Cellulose accounts for the vast majority of the Earth's total biomass and is one of the most promising bioenergy sources existing on Earth (Lynd et al. 2005). Cellulose is a linear polysaccharide chain formed by the oligomerization of glucose units. In organisms, it is mostly cross-linked with hemicellulose and lignin to form a network lattice structure. Degrading cellulose to form glucose and oligosaccharides that can be fermented industrially is the core of cellulose utilization. The enzymatic degradation of cellulose is mainly completed by the synergistic action of exo-cellulase, endo-cellulase, and glucosidase (Lynd et ...

AI Technical Summary

Problems solved by technology

However, most of the cellulase genes that have been cloned are difficult to be applied industrially due to their low activity or poor stability.